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Journal of Clinical Microbiology, March 2000, p. 1032-1035, Vol. 38, No. 3
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Multiplex PCR for Detection of Genes for Staphylococcus aureus Enterotoxins, Exfoliative Toxins, Toxic Shock Syndrome Toxin 1, and Methicillin Resistance

Manisha Mehrotra, Gehua Wang, and Wendy M. Johnson*

Special Project Unit, Bureau of Microbiology, Canadian Science Centre for Human and Animal Health, Winnipeg, Manitoba, Canada

Received 30 August 1999/Returned for modification 27 October 1999/Accepted 5 December 1999

A multiplex PCR assay for detection of genes for staphylococcal enterotoxins A to E (entA, entB, entC, entD, and entE), toxic shock syndrome toxin 1 (tst), exfoliative toxins A and B (etaA and etaB), and intrinsic methicillin resistance (mecA) was developed. Detection of femA was used as an internal positive control. The multiplex PCR assay combined the primers for sea to see and femA in one set and those for eta, etb, tst, mecA, and femA in the other set. Validation of the assay was performed using 176 human isolates of Staphylococcus aureus. This assay offers a very specific, quick, reliable, and inexpensive alternative to conventional PCR assays used in clinical laboratories to identify various staphylococcal toxin genes.

* Corresponding author. Present address: Cangene Corporation, 26 Henlow Bay, Winnipeg, Manitoba, Canada R3Y 1G4. Phone: (204) 275-4301. Fax: (204) 275-4289. E-mail: wjohnson{at}cangene.com.

Journal of Clinical Microbiology, March 2000, p. 1032-1035, Vol. 38, No. 3
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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