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Journal of Clinical Microbiology, March 2000, p. 1042-1047, Vol. 38, No. 3
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Identification and Population Structure of Burkholderia stabilis sp. nov. (formerly Burkholderia cepacia Genomovar IV)

P. Vandamme,1,* E. Mahenthiralingam,2,dagger B. Holmes,3 T. Coenye,1 B. Hoste,1 P. De Vos,1 D. Henry,2 and D. P. Speert2

Laboratorium voor Microbiologie, Faculteit Wetenschappen, Universiteit Gent, Ghent, Belgium1; Department of Pediatrics, Division of Infectious and Immunological Diseases, University of British Columbia and Children's and Women's Health Centre of British Columbia, Vancouver, British Columbia, Canada2; and National Collection of Type Cultures, Central Public Health Laboratory, London NW9 5HT, United Kingdom3

Received 29 June 1999/Returned for modification 23 August 1999/Accepted 7 November 1999

The Burkholderia cepacia complex currently comprises five genomic species, i.e., B. cepacia genomovar I, B. multivorans (formerly known as B. cepacia genomovar II), B. cepacia genomovar III, B. cepacia genomovar IV, and B. vietnamiensis (also known as B. cepacia genomovar V). In the absence of straightforward diagnostic tests for the identification of B. cepacia genomovars I, III, and IV, the last two genomic species were not formally classified as novel Burkholderia species (genomovar I contains the type strain and therefore retains the name B. cepacia). In the present study, we describe differential biochemical tests and a recA gene-based PCR assay for the routine identification of strains currently known as B. cepacia genomovar IV and propose formal classification of this organism as Burkholderia stabilis sp. nov. B. stabilis can indeed be differentiated from all other B. cepacia complex strains by the absence of beta-galactosidase activity, from strains of B. cepacia genomovars I and III and B. vietnamiensis by the inability to oxidize sucrose, and from B. multivorans by the lack of growth at 42°C. In addition, analysis with the recA gene-derived primers BCRG41 (5'-ACCGGCGAGCAGGCGCTT-3') and BCRG42 (5'-ACGCCATCGGGCATGGCA-3') specifically allows the detection of B. stabilis strains in a conventional PCR assay. Examination of a set of 21 B. stabilis strains by means of random amplified polymorphic DNA analysis and pulsed-field gel electrophoresis typing suggested that the genome of this organism is highly conserved, which is in sharp contrast to the generally accepted genomic diversity, variability, and plasticity among B. cepacia strains.

* Corresponding author. Mailing address: Laboratorium voor Microbiologie, Ledeganckstraat 35, B-9000 Ghent, Belgium. Phone: (32)9.264.51.13. Fax: (32)9.264.50.92. E-mail: peter.Vandamme{at}rug.ac.be.

dagger Present address: Cardiff School of Biosciences, Cardiff University, Cardiff, United Kingdom.

Journal of Clinical Microbiology, March 2000, p. 1042-1047, Vol. 38, No. 3
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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