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Journal of Clinical Microbiology, March 2000, p. 1063-1065, Vol. 38, No. 3
Division of Bacteriology, HUCH
Diagnostics,1 and Department of
Obstetrics and Gynecology,3 Helsinki
University Central Hospital, and Department of Bacteriology and
Immunology, Haartman Institute, University of
Helsinki,2 Helsinki, Finland
Received 17 August 1999/Returned for modification 27 September
1999/Accepted 1 December 1999
The Oricult-N semiquantitative dipslide (Orion Diagnostica, Espoo,
Finland) was evaluated for the laboratory diagnosis of vaginal
candidiasis. It was compared with broth culture (Vagicult; Orion
Diagnostica). Oricult-N was positive for 14.5% of 124 symptomatic patients and 12% of 50 asymptomatic controls. The results for broth
cultures were 17 and 22%, respectively. Thus, the test group and the
control group did not differ significantly by either method. High
vaginal yeast counts (
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Evaluation of the Oricult-N Dipslide for Laboratory
Diagnosis of Vaginal Candidiasis
105 CFU/ml) were detected by
Oricult-N in 7% of patients and in 0% of controls, but both groups
harbored low numbers of yeasts. An accurate quantitative cutoff point
separating a level of yeast associated with infection from vaginal
yeast carriage could not be defined in the study. Nevertheless, the
easy semiquantitation allowed by the Oricult-N method could be helpful
because, especially in low-count carriers of Candida, other
potential causes of vaginal symptoms should be considered. The
Oricult-N method was technically simple and could be applied in primary
health care. Further studies are required, however, before Oricult-N
can be recommended as a routine diagnostic tool.
*
Corresponding author. Mailing address: Division of
Bacteriology, HUCH Diagnostics, Helsinki University Central Hospital,
P.O. Box 402, FIN-00029 HYKS, Finland. Phone: 358-9-19126250. Fax: 358-9-19126244. E-mail: petteri.carlson{at}huch.fi.
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