This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pfaller, M. A.
Right arrow Articles by Doern, G. V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pfaller, M. A.
Right arrow Articles by Doern, G. V.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, March 2000, p. 1077-1080, Vol. 38, No. 3
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Evaluation of the Discriminatory Powers of the Dienes Test and Ribotyping as Typing Methods for Proteus mirabilis

M. A. Pfaller,* I. Mujeeb, R. J. Hollis, R. N. Jones, and G. V. Doern

Medical Microbiology Division, Department of Pathology, University of Iowa College of Medicine, Iowa City, Iowa 52242

Received 16 September 1999/Accepted 3 December 1999

A total of 63 clinical isolates of Proteus mirabilis collected over a 19-month period were typed by the Dienes test and ribotyping. Ribotyping was performed using the fully automated RiboPrinter Microbial Characterization System (Qualicon, Wilmington, Del.). Isolates that were indistinguishable by the Dienes test and/or ribotyping were characterized further by pulsed-field gel electrophoresis (PFGE). Most of the isolates represented unique strains as judged by the Dienes test and ribotyping. Forty isolates represented 40 different ribotypes and Dienes types. The remaining 23 isolates were grouped into 13 Dienes types, 12 ribotypes, and 14 PFGE types. The index of discrimination was 0.980 for the Dienes test, 0.979 for ribotyping, and 0.992 for PFGE. Both the Dienes test and ribotyping are useful methods for identifying individual strains of P. mirabilis. The Dienes test is simple, inexpensive, and easy to perform. It can be performed in virtually any laboratory and should be used in the initial epidemiologic characterization of P. mirabilis isolates.

* Corresponding author. Mailing address: Medical Microbiology Division, C606 GH, Department of Pathology, University of Iowa College of Medicine, Iowa City, IA 52242. Phone: (319) 384-9566. Fax: (319) 356-4916. E-mail: michael-pfaller{at}uiowa.edu.

Journal of Clinical Microbiology, March 2000, p. 1077-1080, Vol. 38, No. 3
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Budding, A. E., Ingham, C. J., Bitter, W., Vandenbroucke-Grauls, C. M., Schneeberger, P. M. (2009). The Dienes Phenomenon: Competition and Territoriality in Swarming Proteus mirabilis. J. Bacteriol. 191: 3892-3900 [Abstract] [Full Text]  
  • Sosa, V., Schlapp, G., Zunino, P. (2006). Proteus mirabilis isolates of different origins do not show correlation with virulence attributes and can colonize the urinary tract of mice. Microbiology 152: 2149-2157 [Abstract] [Full Text]  
  • Li, X., Lockatell, C. V., Johnson, D. E., Lane, M. C., Warren, J. W., Mobley, H. L. T. (2004). Development of an Intranasal Vaccine To Prevent Urinary Tract Infection by Proteus mirabilis. Infect. Immun. 72: 66-75 [Abstract] [Full Text]  
  • Nagano, N., Shibata, N., Saitou, Y., Nagano, Y., Arakawa, Y. (2003). Nosocomial Outbreak of Infections by Proteus mirabilis That Produces Extended-Spectrum CTX-M-2 Type {beta}-Lactamase. J. Clin. Microbiol. 41: 5530-5536 [Abstract] [Full Text]  
  • Sabbuba, N. A., Mahenthiralingam, E., Stickler, D. J. (2003). Molecular Epidemiology of Proteus mirabilis Infections of the Catheterized Urinary Tract. J. Clin. Microbiol. 41: 4961-4965 [Abstract] [Full Text]  
  • Cieslikowski, T., Gradecka, D., Mielczarek, M., Kaca, W. (2003). Tandem Tetramer-Based Microsatellite Fingerprinting for Typing of Proteus mirabilis Strains. J. Clin. Microbiol. 41: 1673-1680 [Abstract] [Full Text]  
  • Munson, E. L., Pfaller, M. A., Doern, G. V. (2002). Modification of Dienes Mutual Inhibition Test for Epidemiological Characterization of Pseudomonas aeruginosa Isolates. J. Clin. Microbiol. 40: 4285-4288 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»