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Journal of Clinical Microbiology, March 2000, p. 1144-1150, Vol. 38, No. 3
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Recombinant Antigens To Detect Toxoplasma gondii-Specific Immunoglobulin G and Immunoglobulin M in Human Sera by Enzyme Immunoassay

D. Aubert,1 G. T. Maine,2,* I. Villena,1 J. C. Hunt,2 L. Howard,2 M. Sheu,2 S. Brojanac,2 L. E. Chovan,2 S. F. Nowlan,2 and J. M. Pinon1

Laboratoire de Parasitologie, EA2070, IFR 53 CHU Maison Blanche, 51092 REIMS Cédex, France,1 and Department of Congenital Infectious Disease Diagnostics, Abbott Laboratories, Abbott Park, Illinois2

Received 26 August 1999/Returned for modification 5 November 1999/Accepted 24 December 1999

We have evaluated the diagnostic utility of eleven Toxoplasma gondii recombinant antigens (P22 [SAG2], P24 [GRA1], P25, P28 [GRA2], P29 [GRA7], P30 [SAG1], P35, P41 [GRA4], P54 [ROP2], P66 [ROP1], and P68) in immunoglobulin G (IgG) and IgM recombinant enzyme-linked immunosorbent assays (Rec-ELISAs). Following an initial evaluation, six recombinant antigens (P29, P30, P35, P54, P66, and P68) were tested in the IgG and IgM Rec-ELISAs with four groups of samples which span the toxoplasmosis disease spectrum (negative, chronic infection, acute infection, and recent seroconversion). Our results suggest that the combination of P29, P30, and P35 in an IgG Rec-ELISA and the combination of P29, P35, and P66 in an IgM Rec-ELISA can replace the tachyzoite antigen in IgG and IgM serologic tests, respectively. The relative sensitivity, specificity, and agreement for the IgG P29-P30-P35 Rec-ELISA were 98.4, 95.7, and 97.2%, respectively. The resolved sensitivity, specificity, and agreement for the IgM P29-P35-P66 Rec-ELISA were 93.1, 95.0, and 94.5%, respectively. Relative to the tachyzoite-based immunocapture IgM assay, the IgM P29-P35-P66 Rec-ELISA detects fewer samples that contain IgG antibodies with elevated avidity from individuals with an acute toxoplasmosis.

* Corresponding author. Mailing address: Abbott Laboratories, Bldg. AP31, D-9JW, Abbott Park, IL 60064-6199. Phone: (847) 937-5998. Fax: (847) 938-9219. E-mail: gregory.maine{at}abbott.com.

Journal of Clinical Microbiology, March 2000, p. 1144-1150, Vol. 38, No. 3
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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