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Journal of Clinical Microbiology, March 2000, p. 1238-1240, Vol. 38, No. 3
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Growth of Cowdria ruminantium, the Causative Agent of Heartwater, in a Tick Cell Line

Lesley Bell-Sakyi,1,* Edith A. Paxton,1 Ulrike G. Munderloh,2 and Keith J. Sumption1

Centre for Tropical Veterinary Medicine, University of Edinburgh, Edinburgh, Scotland, United Kingdom,1 and Department of Entomology, University of Minnesota, St. Paul, Minnesota 551082

Received 6 July 1999/Returned for modification 19 October 1999/Accepted 10 December 1999

The tick-borne rickettsia Cowdria ruminantium has been propagated continuously for over 500 days in the Ixodes scapularis tick cell line IDE8 by using the Gardel isolate from bovine endothelial cells as an inoculum. Infection of the tick cells was confirmed by PCR, karyotyping, electron microscopy, and reinfection of bovine cells.

* Corresponding author. Mailing address: Centre for Tropical Veterinary Medicine, University of Edinburgh, Easter Bush, Roslin, Midlothian EH25 9RG, Scotland, United Kingdom. Phone: 44-131-650-8814. Fax: 44-131-445-5099. E-mail: lsakyi{at}ed.ac.uk.

Journal of Clinical Microbiology, March 2000, p. 1238-1240, Vol. 38, No. 3
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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