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Journal of Clinical Microbiology, March 2000, p. 1247-1249, Vol. 38, No. 3
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Use of Calibrated Viral Load Standards for Group M Subtypes of Human Immunodeficiency Virus Type 1 To Assess the Performance of Viral RNA Quantitation Tests

Linda L. Jagodzinski,1,* Debra L. Wiggins,1 Joshua L. McManis,2 Sandra Emery,3 Julie Overbaugh,3 Merlin Robb,2 Sharon Bodrug,4 and Nelson L. Michael2

Henry M. Jackson Foundation1 and Division of Retrovirology, Walter Reed Army Institute of Research,2 Rockville, Maryland 20850; Fred Hutchinson Cancer Research Center, Seattle, Washington 981093; and Gen-Probe, Incorporated, San Diego, California 921214

Received 24 September 1999/Returned for modification 22 November 1999/Accepted 20 December 1999

Optimal management of human immunodeficiency virus type 1 (HIV-1) disease requires accurate quantitation of viral RNA concentrations in plasma. Evidence for increasing geographic intermixing of HIV-1 subtypes makes equivalent quantitation of all subtypes essential. The performances of six quantitative viral RNA tests are described, for the first time, with calibrated viral isolates of diverse subtypes.

* Corresponding author. Mailing address: Henry M. Jackson Foundation, 1600 E. Gude Dr., Rockville, MD 20850. Phone: (301) 251-5042. Fax: (301) 762-7460. E-mail: liagodzinski{at}hiv.hjf.org.

Journal of Clinical Microbiology, March 2000, p. 1247-1249, Vol. 38, No. 3
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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