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Journal of Clinical Microbiology, March 2000, p. 1255-1257, Vol. 38, No. 3
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Rapid Discrimination of Monkey B Virus from Human Herpes Simplex Viruses by PCR in the Presence of Betaine

Makoto Hirano,1 Shin Nakamura,1,* Maki Okada,1 Masahiro Ueda,2 and Ryozaburo Mukai3

Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506,1 Hachioji Laboratory, SRL Co., Ltd., Hachioji, Tokyo 192-8535,2 and Tsukuba Primate Center for Medical Science, National Institute of Infectious Diseases, Tsukuba, Ibaragi 305-0843,3 Japan

Received 15 September 1999/Returned for modification 22 October 1999/Accepted 24 December 1999

A PCR method to amplify DNA segments of the glycoprotein G gene of monkey B virus (BV) was achieved by adding betaine to the PCR mixture, in spite of the high G+C content of this gene. No product was obtained when DNA of human herpes simplex viruses (HSVs) was used as the template under the same conditions. Thus, this PCR method is useful in discriminating BV from HSVs.

* Corresponding author. Mailing address: Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University, Inuyama, Aichi 484-8506, Japan. Phone and fax: 81-(0)-568-63-0579. E-mail: snakamur{at}pri.kyoto-u.ac.jp.

Journal of Clinical Microbiology, March 2000, p. 1255-1257, Vol. 38, No. 3
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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