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Journal of Clinical Microbiology, March 2000, p. 1258-1262, Vol. 38, No. 3
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Molecular Characterization of Brucella Strains Isolated from Marine Mammals

Betsy J. Bricker,1,* Darla R. Ewalt,2 Alastair P. MacMillan,3 Geoff Foster,3 and Simon Brew3

National Animal Disease Center, Agricultural Research Service,1 and National Veterinary Services Laboratories, Animal and Plant Health Inspection Service,2 USDA, Ames, Iowa, and Central Veterinary Laboratory, OIE Brucellosis Reference Laboratory, FAO/WHO Centre for Brucellosis Reference and Research, Weybridge, United Kingdom3

Received 25 May 1999/Returned for modification 22 September 1999/Accepted 30 November 1999

Recently, gram-negative bacteria isolated from a variety of marine mammals have been identified as Brucella species by conventional phenotypic analysis. This study found the 16S rRNA gene from one representative isolate was identical to the homologous sequences of Brucella abortus, B. melitensis, B. canis, and B. suis. IS711-based DNA fingerprinting of 23 isolates from marine mammals showed all the isolates differed from the classical Brucella species. In general, fingerprint patterns grouped by host species. The data suggest that the marine mammal isolates are distinct types of Brucella and not one of the classical species or biovars invading new host species. In keeping with historical precedent, the designation of several new Brucella species may be appropriate.

* Corresponding author. Mailing address: USDA/ARS/National Animal Disease Center, 2300 N. Dayton Ave., P.O. Box 70, Ames, IA 50010. Phone: (515) 663-7310. Fax: (515) 663-7458. E-mail: bbricker{at}nadc.ars.usda.gov.

Journal of Clinical Microbiology, March 2000, p. 1258-1262, Vol. 38, No. 3
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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