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Journal of Clinical Microbiology, March 2000, p. 1286-1289, Vol. 38, No. 3
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Rapid Subtyping of Dengue Virus Serotypes 1 and 4 by Restriction Site-Specific PCR

Marize P. Miagostovich,1 Flavia B. dos Santos,1 C. Milena Gutiérrez,2 Lee W. Riley,3 and Eva Harris3,*

Department of Virology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil1; Department of Virology, Centro Nacional de Diagnóstico y Referencia, Ministry of Health, Managua, Nicaragua2; and Infectious Diseases Unit, Division of Public Health Biology and Epidemiology, School of Public Health, University of California, Berkeley, California3

Received 7 October 1999/Returned for modification 22 November 1999/Accepted 24 December 1999

We previously reported a simple subtyping method, restriction site-specific PCR (RSS-PCR), for dengue virus serotypes 2 and 3; here we describe its application for subtyping dengue virus serotypes 1 and 4. Three major RSS-PCR types were observed for dengue virus serotype 1 and two types were observed for dengue virus serotype 4, in agreement with previous strain classifications based on sequence analysis. Because of its simplicity, this method is amenable to rapid subtyping and application to epidemiological studies of dengue in countries where dengue is endemic.

* Corresponding author. Mailing address: Infectious Diseases Unit, Division of Public Health Biology and Epidemiology, School of Public Health, University of California, Berkeley, 140 Warren Hall, Berkeley, CA 94720-7360. Phone: (510) 642-4845. Fax: (510) 642-6350. E-mail: eharris{at}socrates.berkeley.edu.

Journal of Clinical Microbiology, March 2000, p. 1286-1289, Vol. 38, No. 3
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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Copyright © 2000 by the American Society for Microbiology. All rights reserved.