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Journal of Clinical Microbiology, March 2000, p. 1293-1297, Vol. 38, No. 3
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Helminthic Transmission and Isolation of Ehrlichia risticii, the Causative Agent of Potomac Horse Fever, by Using Trematode Stages from Freshwater Stream Snails

Nicola Pusterla,* John E. Madigan, Joon-Seok Chae, Elfriede DeRock, Eileen Johnson, and Jeannine Berger Pusterla

Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, California 95616

Received 8 October 1999/Returned for modification 5 December 1999/Accepted 30 December 1999

We report successful helminthic transmission of Ehrlichia risticii, the causative agent of Potomac horse fever, using trematode stages collected from Juga yrekaensis snails. The ehrlichial agent was isolated from the blood of experimentally infected horses by culture in murine monocytic cells and identified as E. risticii ultrastructurally and by characterization of three different genes.

* Corresponding author. Mailing address: School of Veterinary Medicine, University of California, Davis, CA 95616. Phone: (530) 752-2371. Fax: (530) 752-0414. E-mail: npusterla{at}ucdavis.edu.

Journal of Clinical Microbiology, March 2000, p. 1293-1297, Vol. 38, No. 3
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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