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Journal of Clinical Microbiology, March 2000, p. 1298-1301, Vol. 38, No. 3
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Genetic and Antigenic Evidence Supports the Separation of Hepatozoon canis and Hepatozoon americanum at the Species Level

Gad Baneth,1,* John R. Barta,2 Varda Shkap,3 Donald S. Martin,2 Douglass K. Macintire,4 and Nancy Vincent-Johnson4,dagger

School of Veterinary Medicine, Hebrew University, Rehovot 76100,1 and Division of Parasitology, Kimron Veterinary Institute, Beit-Dagan 500250,3 Israel; Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario N1G 2W1 Canada2; and College of Veterinary Medicine, Auburn University, Alabama 368494

Received 9 August 1999/Returned for modification 19 October 1999/Accepted 3 December 1999

Recognition of Hepatozoon canis and Hepatozoon americanum as distinct species was supported by the results of Western immunoblotting of canine anti-H. canis and anti-H. americanum sera against H. canis gamonts. Sequence analysis of 368 bases near the 3' end of the 18S rRNA gene from each species revealed a pairwise difference of 13.59%.


* Corresponding author. Mailing address: School of Veterinary Medicine, Hebrew University of Jerusalem, P.O. Box 12, Rehovot 76100, Israel. Phone: 972-3-9688557. Fax: 972-3-9604079. E-mail: baneth{at}agri.huji.ac.il.

dagger Present address: 9723 Flagstaff Dr., San Antonio, TX 78217.


Journal of Clinical Microbiology, March 2000, p. 1298-1301, Vol. 38, No. 3
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.



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