This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Dorigo-Zetsma, J. W.
Right arrow Articles by Zaat, S. A. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dorigo-Zetsma, J. W.
Right arrow Articles by Zaat, S. A. J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, March 2000, p. 965-970, Vol. 38, No. 3
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Genotyping of Mycoplasma pneumoniae Clinical Isolates Reveals Eight P1 Subtypes within Two Genomic Groups

J. Wendelien Dorigo-Zetsma,* Jacob Dankert, and Sebastian A. J. Zaat

Department of Medical Microbiology, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands

Received 20 August 1999/Returned for modification 14 October 1999/Accepted 1 December 1999

Three methods for genotyping of Mycoplasma pneumoniae clinical isolates were applied to 2 reference strains and 21 clinical isolates. By a modified restriction fragment length polymorphism (RFLP) analysis of PCR products of the M. pneumoniae cytadhesin P1 gene, 5 subtypes were discriminated among 13 P1 type 1 strains and 3 subtypes were discriminated among 8 P1 type 2 strains. Sequence analysis of the 16S-23S rRNA gene spacer region and part of the 23S rRNA gene revealed one nucleotide difference in the intergenic spacer region in 3 of the 21 isolates. In the 23S rRNA gene sequence of the 8 P1 type 2 strains an extra adenosine was present, but it was absent from the 13 P1 type 1 strains. On the basis of M. pneumoniae genome sequence data, primers were designed to amplify large interrepeat fragments by long PCR, and these fragments were subsequently analyzed by RFLP analysis. Only two types, long PCR types 1 and 2, could be discriminated among the M. pneumoniae isolates. All P1 type 1 strains were assigned to long PCR type 1, and all P1 type 2 strains were assigned to long PCR type 2. These data obtained by three independent typing methods thus confirm the existence of two distinct M. pneumoniae genomic groups but expand the possibility of strain typing on the basis of variations within their P1 genes.

* Corresponding author. Present address: Diagnostic Laboratory for Infectious Diseases and Perinatal Screening (LIS), National Institute of Public Health and the Environment, Antonie van Leeuwenhoeklaan 9, P.O. Box 1, 3720 BA Bilthoven, The Netherlands. Phone: 31 30 2743705. Fax: 31 30 2744418. E-mail: Wendelien.Dorigo{at}rivm.nl.

Journal of Clinical Microbiology, March 2000, p. 965-970, Vol. 38, No. 3
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Spuesens, E. B. M., Oduber, M., Hoogenboezem, T., Sluijter, M., Hartwig, N. G., van Rossum, A. M. C., Vink, C. (2009). Sequence variations in RepMP2/3 and RepMP4 elements reveal intragenomic homologous DNA recombination events in Mycoplasma pneumoniae. Microbiology 155: 2182-2196 [Abstract] [Full Text]  
  • Maquelin, K., Hoogenboezem, T., Jachtenberg, J.-W., Dumke, R., Jacobs, E., Puppels, G. J., Hartwig, N. G., Vink, C. (2009). Raman spectroscopic typing reveals the presence of carotenoids in Mycoplasma pneumoniae. Microbiology 155: 2068-2077 [Abstract] [Full Text]  
  • Degrange, S., Cazanave, C., Charron, A., Renaudin, H., Bebear, C., Bebear, C. M. (2009). Development of Multiple-Locus Variable-Number Tandem-Repeat Analysis for Molecular Typing of Mycoplasma pneumoniae. J. Clin. Microbiol. 47: 914-923 [Abstract] [Full Text]  
  • Musatovova, O., Kannan, T. R., Baseman, J. B. (2008). Genomic Analysis Reveals Mycoplasma pneumoniae Repetitive Element 1-Mediated Recombination in a Clinical Isolate. Infect. Immun. 76: 1639-1648 [Abstract] [Full Text]  
  • Pereyre, S., Charron, A., Renaudin, H., Bebear, C., Bebear, C. M. (2007). First Report of Macrolide-Resistant Strains and Description of a Novel Nucleotide Sequence Variation in the P1 Adhesin Gene in Mycoplasma pneumoniae Clinical Strains Isolated in France over 12 Years. J. Clin. Microbiol. 45: 3534-3539 [Abstract] [Full Text]  
  • Iverson-Cabral, S. L., Astete, S. G., Cohen, C. R., Rocha, E. P. C., Totten, P. A. (2006). Intrastrain Heterogeneity of the mgpB Gene in Mycoplasma genitalium Is Extensive In Vitro and In Vivo and Suggests that Variation Is Generated via Recombination with Repetitive Chromosomal Sequences. Infect. Immun. 74: 3715-3726 [Abstract] [Full Text]  
  • Dumke, R., Luck, P. C., Noppen, C., Schaefer, C., von Baum, H., Marre, R., Jacobs, E. (2006). Culture-Independent Molecular Subtyping of Mycoplasma pneumoniae in Clinical Samples.. J. Clin. Microbiol. 44: 2567-2570 [Abstract] [Full Text]  
  • Musatovova, O., Herrera, C., Baseman, J. B. (2006). Proximal Region of the Gene Encoding Cytadherence-Related Protein Permits Molecular Typing of Mycoplasma genitalium Clinical Strains by PCR-Restriction Fragment Length Polymorphism. J. Clin. Microbiol. 44: 598-603 [Abstract] [Full Text]  
  • Waites, K. B., Talkington, D. F. (2004). Mycoplasma pneumoniae and Its Role as a Human Pathogen. Clin. Microbiol. Rev. 17: 697-728 [Abstract] [Full Text]  
  • Dorigo-Zetsma, J. W., Wilbrink, B., Dankert, J., Zaat, S. A. J. (2001). Mycoplasma pneumoniae P1 Type 1- and Type 2-Specific Sequences within the P1 Cytadhesin Gene of Individual Strains. Infect. Immun. 69: 5612-5618 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»