This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shibata, S.-i.
Right arrow Articles by Ito, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shibata, S.-i.
Right arrow Articles by Ito, T.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, April 2000, p. 1331-1338, Vol. 38, No. 4
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

New Ehrlichia Species Closely Related to Ehrlichia chaffeensis Isolated from Ixodes ovatus Ticks in Japan

Shin-ichiro Shibata,1 Makoto Kawahara,1 Yasuko Rikihisa,*,2 Hiromi Fujita,3 Yuriko Watanabe,3 Chiharu Suto,4 and Tadahiko Ito5

Nagoya City Public Health Research Institute, Nagoya 467-8615,1 Ohara Research Laboratory, Ohara General Hospital, Fukushima 960-0195,3 Department of Medical Zoology, Nagoya University School of Medicine, Nagoya 466-8550,4 and Tokyo Metropolitan Research Laboratory of Public Health, Tokyo 169-0073,5 Japan, and Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio 43210-10922

Received 6 October 1999/Returned for modification 8 December 1999/Accepted 13 January 2000

Seven Ehrlichia strains (six HF strains and one Anan strain) that were obtained from laboratory mice by intraperitoneally inoculating homogenates of adult Ixodes ovatus collected in Japan were characterized. 16S rRNA sequences of all six HF strains were identical, and the sequences were 99.7, 98.2, and 97.7% identical to those of Anan strain, Ehrlichia chaffeensis (human monocytic ehrlichiosis agent), and E. muris, respectively. Partial GroEL amino acid sequencing also revealed that the six HF strains had identical sequences, which were 99.0, 98.5, and 97.3% identical to those of E. chaffeensis, the Anan strain, and E. canis, respectively. All HF strains were lethal to mice at higher dosages and intraperitoneal inoculation, whereas the Anan or E. muris strain induced only mild clinical signs. Light and electron microscopy of moribund mice inoculated with one of the HF strains revealed severe liver necrosis and the presence of numerous ehrlichial inclusions (morulae) in various organs. The study revealed that members of E. canis genogroup are naturally present in Ixodes ticks. HF strains that can cause severe illness in immunocompetent laboratory mice would be valuable in studying the pathogenesis and the roles of both cellular and humoral immune responses in ehrlichiosis caused by E. canis genogroup.


* Corresponding author. Mailing address: Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, 1925 Coffey Rd., Columbus, OH 43210-1092. Phone: (614) 292-5661. Fax: (614) 292-6473. E-mail: rikihisa.1{at}OSU.edu.


Journal of Clinical Microbiology, April 2000, p. 1331-1338, Vol. 38, No. 4
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.



This article has been cited by other articles:

  • Thirumalapura, N. R., Stevenson, H. L., Walker, D. H., Ismail, N. (2008). Protective Heterologous Immunity against Fatal Ehrlichiosis and Lack of Protection following Homologous Challenge. Infect. Immun. 76: 1920-1930 [Abstract] [Full Text]  
  • Stevenson, H. L., Crossley, E. C., Thirumalapura, N., Walker, D. H., Ismail, N. (2008). Regulatory Roles of CD1d-Restricted NKT Cells in the Induction of Toxic Shock-Like Syndrome in an Animal Model of Fatal Ehrlichiosis. Infect. Immun. 76: 1434-1444 [Abstract] [Full Text]  
  • Nandi, B., Hogle, K., Vitko, N., Winslow, G. M. (2007). CD4 T-Cell Epitopes Associated with Protective Immunity Induced following Vaccination of Mice with an Ehrlichial Variable Outer Membrane Protein. Infect. Immun. 75: 5453-5459 [Abstract] [Full Text]  
  • Ismail, N., Crossley, E. C., Stevenson, H. L., Walker, D. H. (2007). Relative Importance of T-Cell Subsets in Monocytotropic Ehrlichiosis: a Novel Effector Mechanism Involved in Ehrlichia-Induced Immunopathology in Murine Ehrlichiosis. Infect. Immun. 75: 4608-4620 [Abstract] [Full Text]  
  • Miura, K., Rikihisa, Y. (2007). Virulence Potential of Ehrlichia chaffeensis Strains of Distinct Genome Sequences. Infect. Immun. 75: 3604-3613 [Abstract] [Full Text]  
  • Bitsaktsis, C., Winslow, G. (2006). Fatal Recall Responses Mediated by CD8 T cells during Intracellular Bacterial Challenge Infection. J. Immunol. 177: 4644-4651 [Abstract] [Full Text]  
  • Stevenson, H. L., Jordan, J. M., Peerwani, Z., Wang, H.-Q., Walker, D. H., Ismail, N. (2006). An Intradermal Environment Promotes a Protective Type-1 Response against Lethal Systemic Monocytotropic Ehrlichial Infection.. Infect. Immun. 74: 4856-4864 [Abstract] [Full Text]  
  • Kawahara, M., Rikihisa, Y., Lin, Q., Isogai, E., Tahara, K., Itagaki, A., Hiramitsu, Y., Tajima, T. (2006). Novel Genetic Variants of Anaplasma phagocytophilum, Anaplasma bovis, Anaplasma centrale, and a Novel Ehrlichia sp. in Wild Deer and Ticks on Two Major Islands in Japan. Appl. Environ. Microbiol. 72: 1102-1109 [Abstract] [Full Text]  
  • Yager, E., Bitsaktsis, C., Nandi, B., McBride, J. W., Winslow, G. (2005). Essential Role for Humoral Immunity during Ehrlichia Infection in Immunocompetent Mice. Infect. Immun. 73: 8009-8016 [Abstract] [Full Text]  
  • Doyle, C. K., Labruna, M. B., Breitschwerdt, E. B., Tang, Y.-W., Corstvet, R. E., Hegarty, B. C., Bloch, K. C., Li, P., Walker, D. H., McBride, J. W. (2005). Detection of Medically Important Ehrlichia by Quantitative Multicolor TaqMan Real-Time Polymerase Chain Reaction of the dsb Gene. J. Mol. Diagn. 7: 504-510 [Abstract] [Full Text]  
  • Kawahara, M., Rikihisa, Y., Isogai, E., Takahashi, M., Misumi, H., Suto, C., Shibata, S., Zhang, C., Tsuji, M. (2004). Ultrastructure and phylogenetic analysis of 'Candidatus Neoehrlichia mikurensis' in the family Anaplasmataceae, isolated from wild rats and found in Ixodes ovatus ticks. Int. J. Syst. Evol. Microbiol. 54: 1837-1843 [Abstract] [Full Text]  
  • Olano, J. P., Wen, G., Feng, H.-M., McBride, J. W., Walker, D. H. (2004). Histologic, Serologic, and Molecular Analysis of Persistent Ehrlichiosis in a Murine Model. Am. J. Pathol. 165: 997-1006 [Abstract] [Full Text]  
  • Bitsaktsis, C., Huntington, J., Winslow, G. (2004). Production of IFN-{gamma} by CD4 T Cells Is Essential for Resolving Ehrlichia Infection. J. Immunol. 172: 6894-6901 [Abstract] [Full Text]  
  • Inokuma, H., Beppu, T., Okuda, M., Shimada, Y., Sakata, Y. (2004). Detection of Ehrlichial DNA in Haemaphysalis Ticks Recovered from Dogs in Japan That Is Closely Related to a Novel Ehrlichia sp. Found in Cattle Ticks from Tibet, Thailand, and Africa. J. Clin. Microbiol. 42: 1353-1355 [Abstract] [Full Text]  
  • Feng, H.-M., Walker, D. H. (2004). Mechanisms of Immunity to Ehrlichia muris: a Model of Monocytotropic Ehrlichiosis. Infect. Immun. 72: 966-971 [Abstract] [Full Text]  
  • Ismail, N., Soong, L., McBride, J. W., Valbuena, G., Olano, J. P., Feng, H.-M., Walker, D. H. (2004). Overproduction of TNF-{alpha} by CD8+ Type 1 Cells and Down-Regulation of IFN-{gamma} Production by CD4+ Th1 Cells Contribute to Toxic Shock-Like Syndrome in an Animal Model of Fatal Monocytotropic Ehrlichiosis. J. Immunol. 172: 1786-1800 [Abstract] [Full Text]  
  • Park, J.-h., Heo, E.-j., Choi, K.-s., Dumler, J. S., Chae, J.-s. (2003). Detection of Antibodies to Anaplasma phagocytophilum and Ehrlichia chaffeensis Antigens in Sera of Korean Patients by Western Immunoblotting and Indirect Immunofluorescence Assays. CVI 10: 1059-1064 [Abstract] [Full Text]  
  • Parola, P., Cornet, J.-P., Sanogo, Y. O., Miller, R. S., Thien, H. V., Gonzalez, J.-P., Raoult, D., Telford III, S. R., Wongsrichanalai, C. (2003). Detection of Ehrlichia spp., Anaplasma spp., Rickettsia spp., and Other Eubacteria in Ticks from the Thai-Myanmar Border and Vietnam. J. Clin. Microbiol. 41: 1600-1608 [Abstract] [Full Text]  
  • RICHARDS, A. L., RATIWAYANTO, S., RAHARDJO, E., KELLY, D. J., DASCH, G. A., FRYAUFF, D. J., BANGS, M. J. (2003). SEROLOGIC EVIDENCE OF INFECTION WITH EHRLICHIAE AND SPOTTED FEVER GROUP RICKETTSIAE AMONG RESIDENTS OF GAG ISLAND, INDONESIA. Am J Trop Med Hyg 68: 480-484 [Abstract] [Full Text]  
  • Paddock, C. D., Childs, J. E. (2003). Ehrlichia chaffeensis: a Prototypical Emerging Pathogen. Clin. Microbiol. Rev. 16: 37-64 [Abstract] [Full Text]  
  • Wen, B., Jian, R., Zhang, Y., Chen, R. (2002). Simultaneous Detection of Anaplasma marginale and a New Ehrlichia Species Closely Related to Ehrlichia chaffeensis by Sequence Analyses of 16S Ribosomal DNA in Boophilus microplus Ticks from Tibet. J. Clin. Microbiol. 40: 3286-3290 [Abstract] [Full Text]  
  • Heo, E.-j., Park, J.-h., Koo, J.-r., Park, M.-s., Park, M.-y., Dumler, J. S., Chae, J.-s. (2002). Serologic and Molecular Detection of Ehrlichia chaffeensis and Anaplasma phagocytophila (Human Granulocytic Ehrlichiosis Agent) in Korean Patients. J. Clin. Microbiol. 40: 3082-3085 [Abstract] [Full Text]  
  • Inokuma, H., Brouqui, P., Drancourt, M., Raoult, D. (2001). Citrate Synthase Gene Sequence: a New Tool for Phylogenetic Analysis and Identification of Ehrlichia. J. Clin. Microbiol. 39: 3031-3039 [Abstract] [Full Text]  
  • Sotomayor, E. A., Popov, V. L., Feng, H.-M., Walker, D. H., Olano, J. P. (2001). Animal Model of Fatal Human Monocytotropic Ehrlichiosis. Am. J. Pathol. 158: 757-769 [Abstract] [Full Text]  
  • Inokuma, H., Raoult, D., Brouqui, P. (2000). Detection of Ehrlichia platys DNA in Brown Dog Ticks (Rhipicephalus sanguineus) in Okinawa Island, Japan. J. Clin. Microbiol. 38: 4219-4221 [Abstract] [Full Text]