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Journal of Clinical Microbiology, April 2000, p. 1331-1338, Vol. 38, No. 4
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

New Ehrlichia Species Closely Related to Ehrlichia chaffeensis Isolated from Ixodes ovatus Ticks in Japan

Shin-ichiro Shibata,1 Makoto Kawahara,1 Yasuko Rikihisa,*,2 Hiromi Fujita,3 Yuriko Watanabe,3 Chiharu Suto,4 and Tadahiko Ito5

Nagoya City Public Health Research Institute, Nagoya 467-8615,1 Ohara Research Laboratory, Ohara General Hospital, Fukushima 960-0195,3 Department of Medical Zoology, Nagoya University School of Medicine, Nagoya 466-8550,4 and Tokyo Metropolitan Research Laboratory of Public Health, Tokyo 169-0073,5 Japan, and Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio 43210-10922

Received 6 October 1999/Returned for modification 8 December 1999/Accepted 13 January 2000

Seven Ehrlichia strains (six HF strains and one Anan strain) that were obtained from laboratory mice by intraperitoneally inoculating homogenates of adult Ixodes ovatus collected in Japan were characterized. 16S rRNA sequences of all six HF strains were identical, and the sequences were 99.7, 98.2, and 97.7% identical to those of Anan strain, Ehrlichia chaffeensis (human monocytic ehrlichiosis agent), and E. muris, respectively. Partial GroEL amino acid sequencing also revealed that the six HF strains had identical sequences, which were 99.0, 98.5, and 97.3% identical to those of E. chaffeensis, the Anan strain, and E. canis, respectively. All HF strains were lethal to mice at higher dosages and intraperitoneal inoculation, whereas the Anan or E. muris strain induced only mild clinical signs. Light and electron microscopy of moribund mice inoculated with one of the HF strains revealed severe liver necrosis and the presence of numerous ehrlichial inclusions (morulae) in various organs. The study revealed that members of E. canis genogroup are naturally present in Ixodes ticks. HF strains that can cause severe illness in immunocompetent laboratory mice would be valuable in studying the pathogenesis and the roles of both cellular and humoral immune responses in ehrlichiosis caused by E. canis genogroup.

* Corresponding author. Mailing address: Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, 1925 Coffey Rd., Columbus, OH 43210-1092. Phone: (614) 292-5661. Fax: (614) 292-6473. E-mail: rikihisa.1{at}OSU.edu.

Journal of Clinical Microbiology, April 2000, p. 1331-1338, Vol. 38, No. 4
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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