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Journal of Clinical Microbiology, April 2000, p. 1339-1346, Vol. 38, No. 4
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Evaluation of the Wider System, a New
Computer-Assisted Image-Processing Device for Bacterial Identification
and Susceptibility Testing
Rafael
Cantón,*
María
Pérez-Vázquez,
Antonio
Oliver,
Begoña
Sánchez Del Saz,
M. Olga
Gutiérrez,
Manuel
Martínez-Ferrer, and
Fernando
Baquero
Servicio de Microbiología, Hospital
Ramón y Cajal, Madrid 28034, Spain
Received 27 September 1999/Returned for modification 11 November
1999/Accepted 10 January 2000
The Wider system is a newly developed computer-assisted
image-processing device for both bacterial identification and
antimicrobial susceptibility testing. It has been adapted to be able to
read and interpret commercial MicroScan panels. Two hundred forty-four fresh consecutive clinical isolates (138 isolates of the family Enterobacteriaceae, 25 nonfermentative gram-negative rods
[NFGNRs], and 81 gram-positive cocci) were tested. In addition, 100 enterobacterial strains with known
-lactam resistance mechanisms (22 strains with chromosomal AmpC
-lactamase, 8 strains with chromosomal class A
-lactamase, 21 broad-spectrum and IRT
-lactamase-producing strains, 41 extended-spectrum
-lactamase-producing strains, and 8 permeability mutants) were
tested. API galleries and National Committee for Clinical Laboratory
Standards (NCCLS) microdilution methods were used as reference methods.
The Wider system correctly identified 97.5% of the clinical isolates
at the species level. Overall essential agreement (±1 log2
dilution for 3,719 organism-antimicrobial drug combinations) was 95.6%
(isolates of the family Enterobacteriaceae, 96.6%; NFGNRs,
88.0%; gram-positive cocci, 95.6%). The lowest essential agreement
was observed with Enterobacteriaceae versus imipenem
(84.0%), NFGNR versus piperacillin (88.0%) and cefepime (88.0%), and
gram-positive isolates versus penicillin (80.4%). The category error
rate (NCCLS criteria) was 4.2% (2.0% very major errors, 0.6% major
errors, and 1.5% minor errors). Essential agreement and interpretive
error rates for eight
-lactam antibiotics against isolates of the
family Enterobacteriaceae with known
-lactam resistance
mechanisms were 94.8 and 5.4%, respectively. Interestingly, the very
major error rate was only 0.8%. Minor errors (3.6%) were mainly
observed with amoxicillin-clavulanate and cefepime against extended-spectrum
-lactamase-producing isolates. The Wider system is
a new reliable tool which applies the image-processing technology to
the reading of commercial trays for both bacterial identification and
susceptibility testing.
*
Corresponding author. Mailing address: Servicio de
Microbiología, Hospital Ramón y Cajal, Carretera de
Colmenar, Km 9,100, 28034-Madrid, Spain. Phone: 34-91-3368330. Fax:
34-91-3368809. E-mail: rcanton{at}hrc.insalud.es.
Journal of Clinical Microbiology, April 2000, p. 1339-1346, Vol. 38, No. 4
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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