This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cantón, R.
Right arrow Articles by Baquero, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cantón, R.
Right arrow Articles by Baquero, F.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, April 2000, p. 1339-1346, Vol. 38, No. 4
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Evaluation of the Wider System, a New Computer-Assisted Image-Processing Device for Bacterial Identification and Susceptibility Testing

Rafael Cantón,* María Pérez-Vázquez, Antonio Oliver, Begoña Sánchez Del Saz, M. Olga Gutiérrez, Manuel Martínez-Ferrer, and Fernando Baquero

Servicio de Microbiología, Hospital Ramón y Cajal, Madrid 28034, Spain

Received 27 September 1999/Returned for modification 11 November 1999/Accepted 10 January 2000

The Wider system is a newly developed computer-assisted image-processing device for both bacterial identification and antimicrobial susceptibility testing. It has been adapted to be able to read and interpret commercial MicroScan panels. Two hundred forty-four fresh consecutive clinical isolates (138 isolates of the family Enterobacteriaceae, 25 nonfermentative gram-negative rods [NFGNRs], and 81 gram-positive cocci) were tested. In addition, 100 enterobacterial strains with known beta -lactam resistance mechanisms (22 strains with chromosomal AmpC beta -lactamase, 8 strains with chromosomal class A beta -lactamase, 21 broad-spectrum and IRT beta -lactamase-producing strains, 41 extended-spectrum beta -lactamase-producing strains, and 8 permeability mutants) were tested. API galleries and National Committee for Clinical Laboratory Standards (NCCLS) microdilution methods were used as reference methods. The Wider system correctly identified 97.5% of the clinical isolates at the species level. Overall essential agreement (±1 log2 dilution for 3,719 organism-antimicrobial drug combinations) was 95.6% (isolates of the family Enterobacteriaceae, 96.6%; NFGNRs, 88.0%; gram-positive cocci, 95.6%). The lowest essential agreement was observed with Enterobacteriaceae versus imipenem (84.0%), NFGNR versus piperacillin (88.0%) and cefepime (88.0%), and gram-positive isolates versus penicillin (80.4%). The category error rate (NCCLS criteria) was 4.2% (2.0% very major errors, 0.6% major errors, and 1.5% minor errors). Essential agreement and interpretive error rates for eight beta -lactam antibiotics against isolates of the family Enterobacteriaceae with known beta -lactam resistance mechanisms were 94.8 and 5.4%, respectively. Interestingly, the very major error rate was only 0.8%. Minor errors (3.6%) were mainly observed with amoxicillin-clavulanate and cefepime against extended-spectrum beta -lactamase-producing isolates. The Wider system is a new reliable tool which applies the image-processing technology to the reading of commercial trays for both bacterial identification and susceptibility testing.

* Corresponding author. Mailing address: Servicio de Microbiología, Hospital Ramón y Cajal, Carretera de Colmenar, Km 9,100, 28034-Madrid, Spain. Phone: 34-91-3368330. Fax: 34-91-3368809. E-mail: rcanton{at}hrc.insalud.es.

Journal of Clinical Microbiology, April 2000, p. 1339-1346, Vol. 38, No. 4
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Cuenca-Estrella, M., Gomez-Lopez, A., Gutierrez, M. O., Buitrago, M. J., Rodriguez-Tudela, J. L. (2008). Reliability of the WIDERYST Susceptibility Testing System for Detection of In Vitro Antifungal Resistance in Yeasts. Antimicrob. Agents Chemother. 52: 1062-1065 [Abstract] [Full Text]  
  • Gomez-Gonzalez, C., Alba, C., Otero, J. R., Sanz, F., Chaves, F. (2007). Long Persistence of Methicillin-Susceptible Strains of Staphylococcus aureus Causing Sepsis in a Neonatal Intensive Care Unit. J. Clin. Microbiol. 45: 2301-2304 [Abstract] [Full Text]  
  • Mena, A., Plasencia, V., Garcia, L., Hidalgo, O., Ayestaran, J. I., Alberti, S., Borrell, N., Perez, J. L., Oliver, A. (2006). Characterization of a Large Outbreak by CTX-M-1-Producing Klebsiella pneumoniae and Mechanisms Leading to In Vivo Carbapenem Resistance Development.. J. Clin. Microbiol. 44: 2831-2837 [Abstract] [Full Text]  
  • Juan, C., Macia, M. D., Gutierrez, O., Vidal, C., Perez, J. L., Oliver, A. (2005). Molecular Mechanisms of {beta}-Lactam Resistance Mediated by AmpC Hyperproduction in Pseudomonas aeruginosa Clinical Strains. Antimicrob. Agents Chemother. 49: 4733-4738 [Abstract] [Full Text]  
  • Paterson, D. L., Bonomo, R. A. (2005). Extended-Spectrum {beta}-Lactamases: a Clinical Update. Clin. Microbiol. Rev. 18: 657-686 [Abstract] [Full Text]  
  • Chaves, F., Garcia-Alvarez, M., Sanz, F., Alba, C., Otero, J. R. (2005). Nosocomial Spread of a Staphylococcus hominis subsp. novobiosepticus Strain Causing Sepsis in a Neonatal Intensive Care Unit. J. Clin. Microbiol. 43: 4877-4879 [Abstract] [Full Text]  
  • Gutierrez, O., Juan, C., Perez, J. L., Oliver, A. (2004). Lack of Association between Hypermutation and Antibiotic Resistance Development in Pseudomonas aeruginosa Isolates from Intensive Care Unit Patients. Antimicrob. Agents Chemother. 48: 3573-3575 [Abstract] [Full Text]  
  • Rahman, M., Kuhn, I., Rahman, M., Olsson-Liljequist, B., Mollby, R. (2004). Evaluation of a Scanner-Assisted Colorimetric MIC Method for Susceptibility Testing of Gram-Negative Fermentative Bacteria. Appl. Environ. Microbiol. 70: 2398-2403 [Abstract] [Full Text]  
  • Rodriguez, J. C., Sirvent, E., Lopez-Lozano, J. M., Royo, G. (2003). Criteria of time and antibiotic susceptibility in the elimination of duplicates when calculating resistance frequencies. J Antimicrob Chemother 52: 132-134 [Abstract] [Full Text]  
  • Canton, R., Loza, E., Del Carmen Conejo, M., Baquero, F., Martinez-Martinez, L. (2003). Quality Control for {beta}-Lactam Susceptibility Testing with a Well-Defined Collection of Enterobacteriaceae and Pseudomonas aeruginosa Strains in Spain. J. Clin. Microbiol. 41: 1912-1918 [Abstract] [Full Text]  
  • Perez-Perez, F. J., Hanson, N. D. (2002). Detection of Plasmid-Mediated AmpC {beta}-Lactamase Genes in Clinical Isolates by Using Multiplex PCR. J. Clin. Microbiol. 40: 2153-2162 [Abstract] [Full Text]  
  • Gattringer, R., Niks, M., Ostertag, R., Schwarz, K., Medvedovic, H., Graninger, W., Georgopoulos, A. (2002). Evaluation of MIDITECH automated colorimetric MIC reading for antimicrobial susceptibility testing. J Antimicrob Chemother 49: 651-659 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»