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Journal of Clinical Microbiology, April 2000, p. 1397-1403, Vol. 38, No. 4
0095-1137/00/$04.00+0

A Panel of Monoclonal Antibodies Targeting the Rabies Virus Phosphoprotein Identifies a Highly Variable Epitope of Value for Sensitive Strain Discrimination

S. A. Nadin-Davis,* M. Sheen, M. Abdel-Malik, L. Elmgren, J. Armstrong, and A. I. Wandeler

Rabies Centre of Expertise, Animal Diseases Research Institute, Canadian Food Inspection Agency, Nepean, Ontario, Canada, K2H 8P9

Received 30 July 1999/Returned for modification 1 December 1999/Accepted 4 January 2000

A recombinant rabies virus phosphoprotein fusion product (GST-P) was used to generate a series of monoclonal antibodies (MAbs) with anti-P reactivity. Competitive binding assays classified 27 of these MAbs into four groups (I to IV), and 24 of them were deemed to recognize linear epitopes, as judged by their reaction in immunoblots. The linear epitope recognized in each case was mapped by using two series of N- and C-terminally deleted recombinant phosphoproteins. Assessment of the reactivities of representative MAbs to a variety of lyssavirus isolates by an indirect fluorescent antibody test indicated that group I MAbs, which recognized a highly conserved N-terminal epitope, were broadly cross-reactive with all lyssaviruses assayed, while group III MAbs, which reacted with a site overlapping that of group I MAbs, exhibited variable reactivities and group IV MAbs reacted with most isolates of genotypes 1, 6, and 7 only. In contrast, group II MAbs, which recognized an epitope located within a highly divergent central portion of the protein, were exquisitely strain specific. These anti-P MAbs are potentially useful tools for lyssavirus identification and discrimination.

* Corresponding author. Mailing address: Rabies Centre of Expertise, Animal Diseases Research Institute, Canadian Food Inspection Agency, 3851 Fallowfield Rd., Nepean, Ontario K2H 8P9, Canada. Phone: (613) 228-6698. Fax: (613) 228-6669. E-mail: nadindaviss{at}em.agr.ca.

Journal of Clinical Microbiology, April 2000, p. 1397-1403, Vol. 38, No. 4
0095-1137/00/$04.00+0


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