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Journal of Clinical Microbiology, April 2000, p. 1488-1494, Vol. 38, No. 4
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Identification of Campylobacter jejuni, C. coli, C. lari, C. upsaliensis, Arcobacter butzleri, and A. butzleri-Like Species Based on the glyA Gene

Shahnaz Tahihra Al Rashid,1 Irene Dakuna,1 Helena Louie,1 David Ng,1 Peter Vandamme,2 Wendy Johnson,3 and Voon Loong Chan1,*

Department of Medical Genetics and Microbiology, University of Toronto, Ontario, Canada M5S 1A81; Laboratory of Microbiology, University of Ghent, B-9000 Ghent, Belgium2; and Laboratory Centre for Disease Control, Winnipeg, Manitoba, Canada R3E 3R23

Received 9 October 1998/Returned for modification 20 January 1999/Accepted 26 November 1999

Currently, the detection and identification of Campylobacter and Arcobacter species remains arduous, largely due to cross-species phenotypic similarities and a relatively narrow spectrum of biochemical reactivity. We have developed a PCR-hybridization strategy, wherein degenerate primers are used to amplify glyA fragments from samples, which are then subjected to species-specific oligodeoxyribonucleotide probe hybridizations, to identify and distinguish between Campylobacter jejuni, C. coli, C. lari, C. upsaliensis, Arcobacter butzleri, and an A. butzleri-like species. Evaluation of this strategy with genomic DNA from different type strains suggests that this approach is both specific and sensitive and thus may be applicable in a diagnostic assay to identify and differentiate these highly related species.

* Corresponding author. Mailing address: Department of Medical Genetics and Microbiology, Medical Sciences Bldg., University of Toronto, Toronto, Ontario, Canada M5S 1A8. Phone: 416-978-6077. Fax: 416-978-6885. E-mail: voon.chan{at}utoronto.ca.

Journal of Clinical Microbiology, April 2000, p. 1488-1494, Vol. 38, No. 4
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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