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Journal of Clinical Microbiology, April 2000, p. 1520-1526, Vol. 38, No. 4
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Development of a Serological Test for Haemophilus ducreyi for Seroprevalence Studies

Christopher Elkins,1,2,* Kyungcheol Yi,3 Bonnie Olsen,1 Christopher Thomas,1 Kevin Thomas,1 and Stephen Morse3

Departments of Medicine1 and Microbiology and Immunology,2 School of Medicine, University of North Carolina, Chapel Hill, North Carolina, and Centers for Disease Control and Prevention, Atlanta, Georgia3

Received 1 October 1999/Returned for modification 1 December 1999/Accepted 27 January 2000

We developed a new enzyme immunoassay (rpEIA) for use in determining the seroprevalence of chancroid. Three highly conserved outer membrane proteins from Haemophilus ducreyi strain 35000 were cloned, overexpressed, and purified from Escherichia coli for use as antigens in the rpEIA. Serum specimens from patients with and without chancroid were assayed to determine optimum sensitivity and specificity and to establish cutoff values. On the basis of these data, rpEIA was found to be both sensitive and specific when used to test a variety of serum specimens from patients with genital ulcers and urethritis and from healthy blood donors.

* Corresponding author. Mailing address: Departments of Medicine and Microbiology and Immunology, School of Medicine, Room 521, Burnett-Womack, Campus Box 7030, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599. Phone: (919) 966-3661. Fax: (919) 966-6714. E-mail: chriselk{at}med.unc.edu.

Journal of Clinical Microbiology, April 2000, p. 1520-1526, Vol. 38, No. 4
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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