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Journal of Clinical Microbiology, April 2000, p. 1575-1580, Vol. 38, No. 4
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

A Novel Multiresistant Streptococcus pneumoniae Serogroup 19 Clone from Washington State Identified by Pulsed-Field Gel Electrophoresis and Restriction Fragment Length Patterns

Vicki A. Luna,1 Daniel B. Jernigan,2 Alan Tice,3 James D. Kellner,4 and Marilyn C. Roberts1,*

Department of Pathobiology, University of Washington, Seattle,1 and Infections Limited, Tacoma,3 Washington; National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia2; and Departments of Pediatrics and Microbiology and Infectious Diseases, University of Calgary, Calgary, Alberta, Canada4

Received 16 September 1999/Returned for modification 24 December 1999/Accepted 31 January 2000

In 1997, a cluster of multiresistant invasive serogroup 19 pneumococcus infections, including two fatalities, was reported in Washington State. Further investigation identified other cases. Fourteen Washington Streptococcus pneumoniae isolates, four from Alaska, and eight isolates from eastern Canada with reduced penicillin susceptibility (MIC of >= 1 µg/ml) were included in the study. Pulsed-field gel electrophoresis (PFGE) with ApaI, SacII, and SmaI restriction enzymes and IS1167 and mef restriction fragment length polymorphism (RFLP) pattern analysis were performed. Twenty of the 26 isolates had identical or related PFGE patterns, with two or all three enzymes, and identical or related IS1167 RFLP patterns, indicating that they were genetically related. These 20 isolates contained the mef gene conferring erythromycin resistance and had identical mef RFLP patterns. The PFGE and RFLP patterns were distinct from those of six multiresistant clones previously described and suggest that a new multiresistant clone has appeared in Washington, Alaska, and eastern Canada. This newly characterized clone should be included in the Pneumococcal Molecular Epidemiology Network.

* Corresponding author. Mailing address: Department of Pathobiology, Box 357238, School of Public Health and Community Medicine, University of Washington, Seattle, WA 98195-7238. Phone: (206) 543-8001. Fax: (206) 543-3873. E-mail: marilynr{at}u.washington.edu.

Journal of Clinical Microbiology, April 2000, p. 1575-1580, Vol. 38, No. 4
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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