JCM Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Fujimoto, S.
Right arrow Articles by Blaser, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Fujimoto, S.
Right arrow Articles by Blaser, M. J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, April 2000, p. 1664-1667, Vol. 38, No. 4
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Restriction Fragment Length Polymorphism Analysis Using Random Chromosomal Gene Probes for Epidemiological Analysis of Campylobacter jejuni Infections

Shuji Fujimoto,1,* Kenichi Umene,2 Mitsumasa Saito,3 Kazumi Horikawa,4 and Martin J. Blaser5

Infectious Diseases Laboratory, School of Health Science,1 and Department of Virology2 and Department of Bacteriology,3 Faculty of Medicine, Kyushu University, and Fukuoka Institute of Health and Environmental Sciences,4 Fukuoka, Japan, and Division of Infectious Diseases, Vanderbilt University School of Medicine, and Veterans Affairs Medical Center, Nashville, Tennessee5

Received 23 September 1999/Returned for modification 22 November 1999/Accepted 8 January 2000

We have evaluated the ability of a new genotyping method for Campylobacter jejuni based on restriction fragment length polymorphisms using random chromosomal gene probes. DNAs from C. jejuni strains digested with each of three restriction enzymes, HhaI, HaeIII, and HpaII, were analyzed by Southern hybridization using each of two unrelated cosmid clones, P14 and P15 (respectively containing 30- and 35-kb genomic DNA fragments of C. jejuni strain OH4384). The method reported provides a stable and discriminating means for identifying C. jejuni strains and should be useful for epidemiological analyses.

* Corresponding author. Mailing address: Infectious Diseases Laboratory, School of Health Science, Kyushu University, 3-1-1 Maidashi, Higashi-Ku, Fukuoka 812-8582, Japan. Phone: 81-92-642-6732. Fax: 81-92-642-6674. E-mail: fujimoto{at}shs.kyushu-u.ac.jp.

Journal of Clinical Microbiology, April 2000, p. 1664-1667, Vol. 38, No. 4
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.




Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 2000 by the American Society for Microbiology. All rights reserved.