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Journal of Clinical Microbiology, April 2000, p. 1684-1687, Vol. 38, No. 4
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Selective Isolation of eae-Positive Strains of Shiga Toxin-Producing Escherichia coli

Hiroshi Fukushima,* Ken Hoshina, and Manabu Gomyoda

Public Health Institute of Shimane Prefecture, Matsue, Shimane 690-0122, Japan

Received 21 September 1999/Returned for modification 28 September 1999/Accepted 15 January 2000

Culture on cefixime, tellurite, and sorbitol-MacConkey agar after HCl treatment facilitated the growth of 410 (94%) of 436 eae-positive Shiga toxin-producing Escherichia coli (STEC) strains and 17 (16%) of 107 eae-negative STEC strains. This selectivity was closely related to acid resistance in E. coli and tellurite resistance in eae-positive STEC strains.

* Corresponding author. Mailing address: Public Health Institute of Shimane Prefecture, 582-1 Nishihamasada, Matsue, Shimane 690-0122, Japan. Phone: 0852-36-8181. Fax: 0852-36-6683. E-mail: hiroshi{at}joho-shimane.or.jp.

Journal of Clinical Microbiology, April 2000, p. 1684-1687, Vol. 38, No. 4
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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