Distribution of Genes Encoding Putative Transmissibility Factors among Epidemic and Nonepidemic Strains of Burkholderia cepacia from Cystic Fibrosis Patients in the United Kingdom

This Article

	Full Text
	Full Text (PDF)
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager
	Reprints and Permissions
	Copyright Information
	Books from ASM Press
	MicrobeWorld

Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Clode, F. E.
	Articles by Pitt, T. L.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Clode, F. E.
	Articles by Pitt, T. L.

Previous Article | Next Article

Journal of Clinical Microbiology, May 2000, p. 1763-1766, Vol. 38, No. 5
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Distribution of Genes Encoding Putative Transmissibility Factors among Epidemic and Nonepidemic Strains of Burkholderia cepacia from Cystic Fibrosis Patients in the United Kingdom

Fiona E. Clode, Mary E. Kaufmann, Henry Malnick, and Tyrone L. Pitt^*

Laboratory of Hospital Infection, Central Public Health Laboratory, London NW9 5HT, United Kingdom

Received 10 November 1999/Returned for modification 9 January 2000/Accepted 15 February 2000

In the last 15 years, Burkholderia cepacia has emerged as a significant pathogen in cystic fibrosis (CF) patients, mainlydue to the severity of infection observed in a subset of patientsand the fear of transmission of the organism to noncolonized patients.Although patients who deteriorate rapidly cannot be predictedby microbiological characteristics, three genetic markers havebeen described for strains that spread between patients. Theseare the cblA gene, encoding giant cable pili; a hybrid of twoinsertion sequences, IS1356 and IS402; and a 1.4-kb open readingframe known as the B. cepacia epidemic strain marker (BCESM).The latter two are of unknown function. An epidemic strain lineagewas previously identified among CF patients in the United Kingdomthat apparently had spread from North America and that was characterizedby a specific random amplified polymorphic DNA (RAPD) pattern.We searched for the described genetic markers using specific PCRassays with 117 patient isolates of B. cepacia from 40 UnitedKingdom hospitals. Isolates were grouped according to genomovarand epidemic strain lineage RAPD pattern with a 10-base primer,P272. A total of 41 isolates from patients in 12 hospitals wereclassified as the epidemic strain, and 40 of these were distributedin genomovars IIIa (11 isolates), IIIb (1 isolate), and IIIc (28isolates). All isolates of the epidemic strain were positive forthe cblA gene and BCESM, but two lacked the insertion sequencehybrid. None of the 76 sporadic isolates contained cblA or theinsertion sequence hybrid, but 11 of them were positive for BCESM.Nonepidemic isolates were distributed among genomovars I or IV(9), II (49), IIIa (11), IIIb (3), and IIIc (4). There were threeclusters of cross-infection (one involving two patients and twoinvolving three patients) with isolates of genomovar II. We concludethat in the United Kingdom, a single clonal lineage has spreadbetween and within some hospitals providing care for CF patients.The presence of the cblA gene is the most specific marker forthe epidemic strain. We recommend that all isolates of B. cepaciafrom CF patients should be screened by PCR to influence segregationand infection controlstrategies.

^* Corresponding author. Mailing address: Laboratory of Hospital Infection, Central Public Health Laboratory, 61 Colindale Ave.,London NW9 5HT, United Kingdom. Phone: 44 (0)208 200 4400. Fax:44 (0)208 200 7449. E-mail: tpitt{at}phls.nhs.uk.

Journal of Clinical Microbiology, May 2000, p. 1763-1766, Vol. 38, No. 5
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

This article has been cited by other articles:

De Soyza, A., Silipo, A., Lanzetta, R., Govan, J. R., Molinaro, A. (2008). Review: Chemical and biological features of Burkholderia cepacia complex lipopolysaccharides. Innate Immunity 14: 127-144 [Abstract]
Turton, J. F., Arif, N., Hennessy, D., Kaufmann, M. E., Pitt, T. L. (2007). Revised Approach for Identification of Isolates within the Burkholderia cepacia Complex and Description of Clinical Isolates Not Assigned to Any of the Known Genomovars. J. Clin. Microbiol. 45: 3105-3108 [Abstract] [Full Text]
Perin, L., Martinez-Aguilar, L., Castro-Gonzalez, R., Estrada-de los Santos, P., Cabellos-Avelar, T., Guedes, H. V., Reis, V. M., Caballero-Mellado, J. (2006). Diazotrophic burkholderia species associated with field-grown maize and sugarcane.. Appl. Environ. Microbiol. 72: 3103-3110 [Abstract] [Full Text]
Campana, S., Taccetti, G., Ravenni, N., Favari, F., Cariani, L., Sciacca, A., Savoia, D., Collura, A., Fiscarelli, E., De Intinis, G., Busetti, M., Cipolloni, A., d'Aprile, A., Provenzano, E., Collebrusco, I., Frontini, P., Stassi, G., Trancassini, M., Tovagliari, D., Lavitola, A., Doherty, C. J., Coenye, T., Govan, J. R. W., Vandamme, P. (2005). Transmission of Burkholderia cepacia Complex: Evidence for New Epidemic Clones Infecting Cystic Fibrosis Patients in Italy. J. Clin. Microbiol. 43: 5136-5142 [Abstract] [Full Text]
Urban, T. A., Goldberg, J. B., Forstner, J. F., Sajjan, U. S. (2005). Cable Pili and the 22-Kilodalton Adhesin Are Required for Burkholderia cenocepacia Binding to and Transmigration across the Squamous Epithelium. Infect. Immun. 73: 5426-5437 [Abstract] [Full Text]
Brisse, S., Cordevant, C., Vandamme, P., Bidet, P., Loukil, C., Chabanon, G., Lange, M., Bingen, E. (2004). Species Distribution and Ribotype Diversity of Burkholderia cepacia Complex Isolates from French Patients with Cystic Fibrosis. J. Clin. Microbiol. 42: 4824-4827 [Abstract] [Full Text]
Vinion-Dubiel, A. D., Spilker, T., Dean, C. R., Monteil, H., LiPuma, J. J., Goldberg, J. B. (2004). Correlation of wbiI Genotype, Serotype, and Isolate Source within Species of the Burkholderia cepacia Complex. J. Clin. Microbiol. 42: 4121-4126 [Abstract] [Full Text]
Sajjan, U., Keshavjee, S., Forstner, J. (2004). Responses of Well-Differentiated Airway Epithelial Cell Cultures from Healthy Donors and Patients with Cystic Fibrosis to Burkholderia cenocepacia Infection. Infect. Immun. 72: 4188-4199 [Abstract] [Full Text]
McDowell, A., Mahenthiralingam, E., Dunbar, K. E.A., Moore, J. E., Crowe, M., Elborn, J. S. (2004). Epidemiology of Burkholderia cepacia complex species recovered from cystic fibrosis patients: issues related to patient segregation. J Med Microbiol 53: 663-668 [Abstract] [Full Text]
De Boeck, K., Malfroot, A., Van Schil, L., Lebecque, P., Knoop, C., Govan, J.R.W., Doherty, C., Laevens, S., Vandamme, P. (2004). Epidemiology of Burkholderia cepacia complex colonisation in cystic fibrosis patients. Eur Respir J 23: 851-856 [Abstract] [Full Text]
Plesa, M., Kholti, A., Vermis, K., Vandamme, P., Panagea, S., Winstanley, C., Cornelis, P. (2004). Conservation of the opcL gene encoding the peptidoglycan-associated outer-membrane lipoprotein among representatives of the Burkholderia cepacia complex. J Med Microbiol 53: 389-398 [Abstract] [Full Text]
Manno, G., Dalmastri, C., Tabacchioni, S., Vandamme, P., Lorini, R., Minicucci, L., Romano, L., Giannattasio, A., Chiarini, L., Bevivino, A. (2004). Epidemiology and Clinical Course of Burkholderia cepacia Complex Infections, Particularly Those Caused by Different Burkholderia cenocepacia Strains, among Patients Attending an Italian Cystic Fibrosis Center. J. Clin. Microbiol. 42: 1491-1497 [Abstract] [Full Text]
Tomich, M., Mohr, C. D. (2004). Transcriptional and Posttranscriptional Control of Cable Pilus Gene Expression in Burkholderia cenocepacia. J. Bacteriol. 186: 1009-1020 [Abstract] [Full Text]
Cunha, M. V., Leitao, J. H., Mahenthiralingam, E., Vandamme, P., Lito, L., Barreto, C., Salgado, M. J., Sa-Correia, I. (2003). Molecular Analysis of Burkholderia cepacia Complex Isolates from a Portuguese Cystic Fibrosis Center: a 7-Year Study. J. Clin. Microbiol. 41: 4113-4120 [Abstract] [Full Text]
Detsika, M. G., Corkill, J. E., Magalhaes, M., Glendinning, K. J., Hart, C. A., Winstanley, C. (2003). Molecular Typing of, and Distribution of Genetic Markers among, Burkholderia cepacia Complex Isolates from Brazil. J. Clin. Microbiol. 41: 4148-4153 [Abstract] [Full Text]
Vinion-Dubiel, A. D., Goldberg, J. B. (2003). Review: Lipopolysaccharide of Burkholderia cepacia complex. Innate Immunity 9: 201-213 [Abstract]
Coenye, T., LiPuma, J. J. (2003). Population structure analysis of Burkholderia cepacia genomovar III: varying degrees of genetic recombination characterize major clonal complexes. Microbiology 149: 77-88 [Abstract] [Full Text]
Sajjan, U., Liu, L., Lu, A., Spilker, T., Forstner, J., LiPuma, J. J. (2002). Lack of cable pili expression by cblA-containing Burkholderia cepacia complex. Microbiology 148: 3477-3484 [Abstract] [Full Text]
CANT, A.J., GORDON, S.B., READ, R.C., HART, C.A., WINSTANLEY, C. (2002). Respiratory infections: Proceedings of the Eighth Liverpool Tropical School Bayer Symposium of Microbial Disease held on 3 February 2001. J Med Microbiol 51: 903-914 [Full Text]
Heath, D. G., Hohneker, K., Carriker, C., Smith, K., Routh, J., LiPuma, J. J., Aris, R. M., Weber, D., Gilligan, P. H. (2002). Six-Year Molecular Analysis of Burkholderia cepacia Complex Isolates among Cystic Fibrosis Patients at a Referral Center for Lung Transplantation. J. Clin. Microbiol. 40: 1188-1193 [Abstract] [Full Text]
Bevivino, A., Dalmastri, C., Tabacchioni, S., Chiarini, L., Belli, M. L., Piana, S., Materazzo, A., Vandamme, P., Manno, G. (2002). Burkholderia cepacia Complex Bacteria from Clinical and Environmental Sources in Italy: Genomovar Status and Distribution of Traits Related to Virulence and Transmissibility. J. Clin. Microbiol. 40: 846-851 [Abstract] [Full Text]
Hart, C A., Winstanley, C. (2002). Persistent and aggressive bacteria in the lungs of cystic fibrosis children. Br Med Bull 61: 81-96 [Abstract] [Full Text]
ARIS, R. M., ROUTH, J. C., LIPUMA, J. J., HEATH, D. G., GILLIGAN, P. H. (2001). Lung Transplantation for Cystic Fibrosis Patients with Burkholderia cepacia Complex . Survival Linked to Genomovar Type. Am. J. Respir. Crit. Care Med. 164: 2102-2106 [Abstract] [Full Text]
Agodi, A., Mahenthiralingam, E., Barchitta, M., Giannino, V., Sciacca, A., Stefani, S. (2001). Burkholderia cepacia Complex Infection in Italian Patients with Cystic Fibrosis: Prevalence, Epidemiology, and Genomovar Status. J. Clin. Microbiol. 39: 2891-2896 [Abstract] [Full Text]
Lutter, E., Lewenza, S., Dennis, J. J., Visser, M. B., Sokol, P. A. (2001). Distribution of Quorum-Sensing Genes in the Burkholderia cepacia Complex. Infect. Immun. 69: 4661-4666 [Abstract] [Full Text]
LiPUMA, J. J., SPILKER, T., GILL, L. H., CAMPBELL, P. W. III, LIU, L., MAHENTHIRALINGAM, E. (2001). Disproportionate Distribution of Burkholderia cepacia Complex Species and Transmissibility Markers in Cystic Fibrosis. Am. J. Respir. Crit. Care Med. 164: 92-96 [Abstract] [Full Text]
Henry, D. A., Mahenthiralingam, E., Vandamme, P., Coenye, T., Speert, D. P. (2001). Phenotypic Methods for Determining Genomovar Status of the Burkholderia cepacia Complex. J. Clin. Microbiol. 39: 1073-1078 [Abstract] [Full Text]

Antimicrob. Agents Chemother.	Clin. Microbiol. Rev.

Clin. Vaccine Immunol.	ALL ASM JOURNALS