This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yarwood, J. M.
Right arrow Articles by Schlievert, P. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yarwood, J. M.
Right arrow Articles by Schlievert, P. M.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, May 2000, p. 1797-1803, Vol. 38, No. 5
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Oxygen and Carbon Dioxide Regulation of Toxic Shock Syndrome Toxin 1 Production by Staphylococcus aureus MN8

Jeremy M. Yarwood and Patrick M. Schlievert*

Department of Microbiology, University of Minnesota Medical School, Minneapolis, Minnesota 55455

Received 16 July 1999/Returned for modification 19 November 1999/Accepted 11 February 2000

The production of toxic shock syndrome toxin 1 (TSST-1) by Staphylococcus aureus MN8 exposed to a range of oxygen concentrations (0 to 21% [vol/vol]) was examined in batch and thin-film cultures. The response of S. aureus to this range of oxygen concentrations was studied in the absence and in the presence of 7% (vol/vol) carbon dioxide. In the absence of carbon dioxide, TSST-1 production in batch cultures increased from negligible levels in the presence of oxygen concentrations of 1% or less to 500 ng/ml in the presence of 2% oxygen and then decreased to 70 ng/ml or less in the presence of oxygen concentrations of 6% and higher. In the presence of carbon dioxide, however, toxin production increased from negligible levels in the presence of 1% oxygen to 1,900 ng/ml in the presence of 21% oxygen. In thin-film cultures, TSST-1 production increased from nearly undetectable levels under anaerobic conditions to 1 and 10 µg/ml under 21% oxygen in the absence and presence of carbon dioxide, respectively. This study demonstrates the controlling effects of both oxygen and carbon dioxide on TSST-1 production.

* Corresponding author. Mailing address: Department of Microbiology, Medical School, University of Minnesota, Box 196 FUMC, 420 Delaware St. SE, Minneapolis, MN 55455. Phone: (612) 624-9471. Fax: (612) 626-0623. E-mail: pats{at}lenti.med.umn.edu.

Journal of Clinical Microbiology, May 2000, p. 1797-1803, Vol. 38, No. 5
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Seidl, K., Bischoff, M., Berger-Bachi, B. (2008). CcpA Mediates the Catabolite Repression of tst in Staphylococcus aureus. Infect. Immun. 76: 5093-5099 [Abstract] [Full Text]  
  • Schlievert, P. M. (2007). Chitosan Malate Inhibits Growth and Exotoxin Production of Toxic Shock Syndrome-Inducing Staphylococcus aureus Strains and Group A Streptococci. Antimicrob. Agents Chemother. 51: 3056-3062 [Abstract] [Full Text]  
  • Cook, E., Wang, X., Robiou, N., Fries, B. C. (2007). Measurement of Staphylococcal Enterotoxin B in Serum and Culture Supernatant with a Capture Enzyme-Linked Immunosorbent Assay. CVI 14: 1094-1101 [Abstract] [Full Text]  
  • Fuchs, S., Pane-Farre, J., Kohler, C., Hecker, M., Engelmann, S. (2007). Anaerobic Gene Expression in Staphylococcus aureus. J. Bacteriol. 189: 4275-4289 [Abstract] [Full Text]  
  • Hill, D. R., Brunner, M. E., Schmitz, D. C., Davis, C. C., Flood, J. A., Schlievert, P. M., Wang-Weigand, S. Z., Osborn, T. W. (2005). In vivo assessment of human vaginal oxygen and carbon dioxide levels during and post menses. J. Appl. Physiol. 99: 1582-1591 [Abstract] [Full Text]  
  • Parsonnet, J., Hansmann, M. A., Delaney, M. L., Modern, P. A., DuBois, A. M., Wieland-Alter, W., Wissemann, K. W., Wild, J. E., Jones, M. B., Seymour, J. L., Onderdonk, A. B. (2005). Prevalence of Toxic Shock Syndrome Toxin 1-Producing Staphylococcus aureus and the Presence of Antibodies to This Superantigen in Menstruating Women. J. Clin. Microbiol. 43: 4628-4634 [Abstract] [Full Text]  
  • Peterson, M. L., Ault, K., Kremer, M. J., Klingelhutz, A. J., Davis, C. C., Squier, C. A., Schlievert, P. M. (2005). The Innate Immune System Is Activated by Stimulation of Vaginal Epithelial Cells with Staphylococcus aureus and Toxic Shock Syndrome Toxin 1. Infect. Immun. 73: 2164-2174 [Abstract] [Full Text]  
  • Pragman, A. A., Yarwood, J. M., Tripp, T. J., Schlievert, P. M. (2004). Characterization of Virulence Factor Regulation by SrrAB, a Two-Component System in Staphylococcus aureus. J. Bacteriol. 186: 2430-2438 [Abstract] [Full Text]  
  • Yarwood, J. M., Bartels, D. J., Volper, E. M., Greenberg, E. P. (2004). Quorum Sensing in Staphylococcus aureus Biofilms. J. Bacteriol. 186: 1838-1850 [Abstract] [Full Text]  
  • Kikuchi, K., Takahashi, N., Piao, C., Totsuka, K., Nishida, H., Uchiyama, T. (2003). Molecular Epidemiology of Methicillin-Resistant Staphylococcus aureus Strains Causing Neonatal Toxic Shock Syndrome-Like Exanthematous Disease in Neonatal and Perinatal Wards. J. Clin. Microbiol. 41: 3001-3006 [Abstract] [Full Text]  
  • Yarwood, J. M., McCormick, J. K., Paustian, M. L., Orwin, P. M., Kapur, V., Schlievert, P. M. (2002). Characterization and Expression Analysis of Staphylococcus aureus Pathogenicity Island 3. IMPLICATIONS FOR THE EVOLUTION OF STAPHYLOCOCCAL PATHOGENICITY ISLANDS. J. Biol. Chem. 277: 13138-13147 [Abstract] [Full Text]  
  • Yarwood, J. M., McCormick, J. K., Paustian, M. L., Kapur, V., Schlievert, P. M. (2002). Repression of the Staphylococcus aureus Accessory Gene Regulator in Serum and In Vivo. J. Bacteriol. 184: 1095-1101 [Abstract] [Full Text]  
  • Masalha, M., Borovok, I., Schreiber, R., Aharonowitz, Y., Cohen, G. (2001). Analysis of Transcription of the Staphylococcus aureus Aerobic Class Ib and Anaerobic Class III Ribonucleotide Reductase Genes in Response to Oxygen. J. Bacteriol. 183: 7260-7272 [Abstract] [Full Text]  
  • Yarwood, J. M., McCormick, J. K., Schlievert, P. M. (2001). Identification of a Novel Two-Component Regulatory System That Acts in Global Regulation of Virulence Factors of Staphylococcus aureus. J. Bacteriol. 183: 1113-1123 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»