Previous Article | Next Article ![]()
Journal of Clinical Microbiology, May 2000, p. 1827-1831, Vol. 38, No. 5
Division of Vector-Borne Infectious Diseases,
National Center for Infectious Diseases, Centers for Disease Control
and Prevention, Public Health Service, U.S. Department of Health and
Human Services, Fort Collins, Colorado 80522
Received 3 August 1999/Returned for modification 1 November
1999/Accepted 14 February 2000
Monoclonal antibody (MAb)-based capture enzyme-linked immunosorbent
assays (ELISAs) for the detection of anti-arboviral immunoglobulin G
(IgG ELISAs) were developed for a comprehensive array of medically important arboviruses from the Alphavirus,
Flavivirus, and Bunyavirus genera. Tests were
optimized and standardized so that maximum homology could be
maintained among working parameters for the different viral agents,
enabling a wide range of viruses to be easily tested for at one time.
MAbs were screened for suitability as capture vehicles for antigens
from the three genera. The final test configuration utilized
group-reactive MAbs eastern equine encephalitis virus 1A4B-6, dengue 2 virus 4G2, and La Crosse encephalitis virus 10G5.4 to capture the
specific inactivated viral antigens. Serum IgG was detected by using
alkaline phosphatase-conjugated anti-human IgG (Fc portion). A
dilution of 1:400 was chosen as the universal screening serum dilution,
with endpoint titrations of serum samples testing positive eliminating
occasional false-positive results. IgG ELISA results correlated with
those of the standard plaque-reduction neutralization assays. As
expected, some test cross-reactivity was encountered within the
individual genera, and tests were interpreted within the context of
these reactions. The tests were standardized for laboratory diagnosis
of arboviral infections, with the intent that they be used in tandem
with the corresponding IgM antibody-capture ELISAs.
0095-1137/00/$04.00+0
Detection of Anti-Arboviral Immunoglobulin G by
Using a Monoclonal Antibody-Based Capture Enzyme-Linked
Immunosorbent Assay
*
Corresponding author. Mailing address: Division of
Vector-Borne Infectious Diseases, National Center for Infectious
Diseases, Centers for Disease Control and Prevention, P.O. Box 2087, Fort Collins, CO 80522. Phone: (970) 221-6469. Fax: (970) 221-6476. E-mail: AJJ1{at}CDC.GOV.
This article has been cited by other articles:
| Antimicrob. Agents Chemother. | Clin. Microbiol. Rev. |
|---|---|
| Clin. Vaccine Immunol. | ALL ASM JOURNALS |
|---|