This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Miller, N.
Right arrow Articles by Cleary, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Miller, N.
Right arrow Articles by Cleary, T.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, May 2000, p. 1915-1919, Vol. 38, No. 5
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Evaluation of the LiPA MYCOBACTERIA Assay for Identification of Mycobacterial Species from BACTEC 12B Bottles

Nancimae Miller,* Susanna Infante, and Tim Cleary

Department of Pathology, Jackson Memorial Medical Center, University of Miami, Miami, Florida

Received 22 November 1999/Returned for modification 22 January 2000/Accepted 28 February 2000

The LiPA MYCOBACTERIA (Innogenetics NV, Ghent, Belgium) assay was used to identify mycobacterial isolates using culture fluid from positive BACTEC 12B bottles. The LiPA method involves reverse hybridization of a biotinylated mycobacterial PCR fragment, a 16 to 23S rRNA spacer region, to oligonucleotide probes arranged in lines on a membrane strip, with detection via biotin-streptavidin coupling by a colorimetric system. This system identifies Mycobacterium species and differentiates M. tuberculosis complex, M. avium-M. intracellulare complex, and the following mycobacterial species: M. avium, M. intracellulare, M. kansasii, M. chelonae group, M. gordonae, M. xenopi, and M. scrofulaceum. The mycobacteria were identified in the laboratory by a series of tests, including the Roche AMPLICOR Mycobacterium tuberculosis (MTB) test, the Gen-Probe ACCUPROBE, and a PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the 65-kDa heat shock protein gene. The LiPA MYCOBACTERIA assay detected 60 mycobacterium isolates from 59 patients. There was complete agreement between LiPA and the laboratory identification tests for 26 M. tuberculosis complex, 9 M. avium, 3 M. intracellulare complex, 3 M. kansasii, 4 M. gordonae, and 5 M. chelonae group (all were M. abscessus) isolates. Three patient samples were LiPA positive for M. avium-M. intracellulare complex, and all were identified as M. intracellulare by the PCR-RFLP analysis. Seven additional mycobacterial species were LiPA positive for Mycobacterium spp. (six were M. fortuitum, and one was M. szulgai). The LiPA MYCOBACTERIA assay was easy to perform, and the interpretation of the positive bands was clear-cut. Following PCR amplification and gel electrophoresis, the LiPA assay was completed within 3 h.

* Corresponding author. Mailing address: Department of Pathology, University of Miami, P.O. Box 016960, Miami, FL 33101. Phone: (305) 585-6258. Fax: (305) 585-0008. E-mail: nmiller{at}med.miami.edu.

Journal of Clinical Microbiology, May 2000, p. 1915-1919, Vol. 38, No. 5
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Pignone, M., Greth, K. M., Cooper, J., Emerson, D., Tang, J. (2006). Identification of Mycobacteria by Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry.. J. Clin. Microbiol. 44: 1963-1970 [Abstract] [Full Text]  
  • Suffys, P., Rocha, A. d. S., Brandao, A., Vanderborght, B., Mijs, W., Jannes, G., Mello, F. C. Q., Pedro, H. d. S. P., de Souza Fonseca, L., de Oliveira, R. S., Leao, S. C., Saad, M. H. F. (2006). Detection of mixed infections with Mycobacterium lentiflavum and Mycobacterium avium by molecular genotyping methods. J Med Microbiol 55: 127-131 [Abstract] [Full Text]  
  • Palomino, J. C. (2005). Nonconventional and new methods in the diagnosis of tuberculosis: feasibility and applicability in the field. Eur Respir J 26: 339-350 [Abstract] [Full Text]  
  • Tortoli, E., Rindi, L., Garcia, M. J., Chiaradonna, P., Dei, R., Garzelli, C., Kroppenstedt, R. M., Lari, N., Mattei, R., Mariottini, A., Mazzarelli, G., Murcia, M. I., Nanetti, A., Piccoli, P., Scarparo, C. (2004). Proposal to elevate the genetic variant MAC-A, included in the Mycobacterium avium complex, to species rank as Mycobacterium chimaera sp. nov.. Int. J. Syst. Evol. Microbiol. 54: 1277-1285 [Abstract] [Full Text]  
  • Padilla, E., Gonzalez, V., Manterola, J. M., Perez, A., Quesada, M. D., Gordillo, S., Vilaplana, C., Pallares, M. A., Molinos, S., Sanchez, M. D., Ausina, V. (2004). Comparative Evaluation of the New Version of the INNO-LiPA Mycobacteria and GenoType Mycobacterium Assays for Identification of Mycobacterium Species from MB/BacT Liquid Cultures Artificially Inoculated with Mycobacterial Strains. J. Clin. Microbiol. 42: 3083-3088 [Abstract] [Full Text]  
  • Lefmann, M., Honisch, C., Bocker, S., Storm, N., von Wintzingerode, F., Schlotelburg, C., Moter, A., van den Boom, D., Gobel, U. B. (2004). Novel Mass Spectrometry-Based Tool for Genotypic Identification of Mycobacteria. J. Clin. Microbiol. 42: 339-346 [Abstract] [Full Text]  
  • Shrestha, N. K., Tuohy, M. J., Hall, G. S., Reischl, U., Gordon, S. M., Procop, G. W. (2003). Detection and Differentiation of Mycobacterium tuberculosis and Nontuberculous Mycobacterial Isolates by Real-Time PCR. J. Clin. Microbiol. 41: 5121-5126 [Abstract] [Full Text]  
  • Tortoli, E., Mariottini, A., Mazzarelli, G. (2003). Evaluation of INNO-LiPA MYCOBACTERIA v2: Improved Reverse Hybridization Multiple DNA Probe Assay for Mycobacterial Identification. J. Clin. Microbiol. 41: 4418-4420 [Abstract] [Full Text]  
  • Dauendorffer, J.-N., Guillemin, I., Aubry, A., Truffot-Pernot, C., Sougakoff, W., Jarlier, V., Cambau, E. (2003). Identification of Mycobacterial Species by PCR Sequencing of Quinolone Resistance-Determining Regions of DNA Gyrase Genes. J. Clin. Microbiol. 41: 1311-1315 [Abstract] [Full Text]  
  • da Silva Rocha, A., Werneck Barreto, A. M., Dias Campos, C. E., Villas-Boas da Silva, M., Fonseca, L., Saad, M. H., Degrave, W. M., Suffys, P. N. (2002). Novel Allelic Variants of Mycobacteria Isolated in Brazil as Determined by PCR-Restriction Enzyme Analysis of hsp65. J. Clin. Microbiol. 40: 4191-4196 [Abstract] [Full Text]  
  • Makinen, J., Sarkola, A., Marjamaki, M., Viljanen, M. K., Soini, H. (2002). Evaluation of GenoType and LiPA MYCOBACTERIA Assays for Identification of Finnish Mycobacterial Isolates. J. Clin. Microbiol. 40: 3478-3481 [Abstract] [Full Text]  
  • Ruiz, P., Gutierrez, J., Zerolo, F. J., Casal, M. (2002). GenoType Mycobacterium Assay for Identification of Mycobacterial Species Isolated from Human Clinical Samples by Using Liquid Medium. J. Clin. Microbiol. 40: 3076-3078 [Abstract] [Full Text]  
  • Suffys, P. N., da Silva Rocha, A., de Oliveira, M., Dias Campos, C. E., Werneck Barreto, A. M., Portaels, F., Rigouts, L., Wouters, G., Jannes, G., van Reybroeck, G., Mijs, W., Vanderborght, B. (2001). Rapid Identification of Mycobacteria to the Species Level Using INNO-LiPA Mycobacteria, a Reverse Hybridization Assay. J. Clin. Microbiol. 39: 4477-4482 [Abstract] [Full Text]  
  • Scarparo, C., Piccoli, P., Rigon, A., Ruggiero, G., Nista, D., Piersimoni, C. (2001). Direct Identification of Mycobacteria from MB/BacT Alert 3D Bottles: Comparative Evaluation of Two Commercial Probe Assays. J. Clin. Microbiol. 39: 3222-3227 [Abstract] [Full Text]  
  • Tortoli, E., Nanetti, A., Piersimoni, C., Cichero, P., Farina, C., Mucignat, G., Scarparo, C., Bartolini, L., Valentini, R., Nista, D., Gesu, G., Tosi, C. P., Crovatto, M., Brusarosco, G. (2001). Performance Assessment of New Multiplex Probe Assay for Identification of Mycobacteria. J. Clin. Microbiol. 39: 1079-1084 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»