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Journal of Clinical Microbiology, May 2000, p. 1977-1980, Vol. 38, No. 5
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Evaluation of Quantitative PCR and Branched-Chain DNA Assay for Detection of Hepatitis B Virus DNA in Sera from Hepatocellular Carcinoma and Liver Transplant Patients

Tao Chen, John M. Luk,* Siu-Tim Cheung, Wan-Ching Yu, and Sheung-Tat Fan

Center of Liver Diseases and Department of Surgery, University of Hong Kong Medical Center, Queen Mary Hospital, Pokfulam, Hong Kong

Received 8 November 1999/Returned for modification 19 January 2000/Accepted 21 February 2000

This study evaluated the applicability of quantitative PCR (Q-PCR) and branched-chain DNA assays for detection of hepatitis B virus (HBV) DNA in sera. For 42 samples, the detection rates were 81 and 41%, respectively, with a correlation coefficient of 0.633. The Q-PCR is useful for early monitoring of HBV load in high-risk patients.

* Corresponding author. Present address: Division of Rheumatology, Immunology & Allergy, Brigham & Women's Hospital, 1 Jimmy Fund Way, SM-624, Boston, MA 02115. Phone: (617) 525-1251. Fax: (617) 525-1310. E-mail: jmluk{at}hkucc.hku.hk.

Journal of Clinical Microbiology, May 2000, p. 1977-1980, Vol. 38, No. 5
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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