JCM Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zerr, D. M.
Right arrow Articles by Frenkel, L. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zerr, D. M.
Right arrow Articles by Frenkel, L. M.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, May 2000, p. 1981-1983, Vol. 38, No. 5
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Sensitive Method for Detection of Human Herpesviruses 6 and 7 in Saliva Collected in Field Studies

Danielle M. Zerr,1,* Meei-Li Huang,2 Lawrence Corey,2,3 Matthew Erickson,3 Heather L. Parker,3 and Lisa M. Frenkel1,2

Departments of Pediatrics1 and Laboratory Medicine,2 University of Washington, and Department of Infectious Diseases, Fred Hutchinson Cancer Research Center,3 Seattle, Washington

Received 27 May 1999/Returned for modification 25 August 1999/Accepted 4 November 1999

To facilitate studies of the epidemiology and natural history of human herpesviruses 6 and 7 in infants, a practical method for collecting and quantifying the DNA of these viruses was developed. Saliva was collected using small strips of filter paper, and virus was detected using a real-time quantitative fluorescent-probe PCR assay. The sensitivity and specificity of this method even after prolonged drying of the specimens compared favorably to those of our traditional method of collecting and assaying saliva.

* Corresponding author. Mailing address: Children's Hospital and Regional Medical Center, CH-32, 4800 Sand Point Way, N.E., Seattle, WA 98105. Phone: (206) 528-5086. Fax: (206) 527-3897. E-mail: zerr{at}u.washington.edu.

Journal of Clinical Microbiology, May 2000, p. 1981-1983, Vol. 38, No. 5
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 2000 by the American Society for Microbiology. All rights reserved.