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Journal of Clinical Microbiology, May 2000, p. 1984-1987, Vol. 38, No. 5
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Growth Supplements for Helicobacter pylori

Xiuping Jiang and Michael P. Doyle*

Center for Food Safety and Quality Enhancement and Department of Food Science and Technology, University of Georgia, Griffin, Georgia 30223-1797

Received 26 July 1999/Returned for modification 9 December 1999/Accepted 2 February 2000

The growth response of Helicobacter pylori in broth was determined in the presence of ferrous sulfate, sodium pyruvate, and mucin (porcine stomach). The addition of either ferrous sulfate and sodium pyruvate or mucin to brain heart infusion broth with 7% horse serum (BHI-HS) enhanced the growth of H. pylori. The best growth of strain NB2-1, which was the slowest growing of 10 H. pylori strains evaluated, occurred in the presence of 0.05% ferrous sulfate and 0.05% sodium pyruvate. The addition of 0.3% mucin to BHI-HS reduced the lag time of H. pylori by 48 h and enhanced the growth. On the basis of the results for 10 H. pylori strains, the combination of ferrous sulfate (0.025%), sodium pyruvate (0.025%), and mucin (0.15%) in BHI-HS counteracted the inhibitory effects of the antibiotics used in culture media for selective growth of H. pylori. Results suggest that these supplements may be useful for enhancement of the growth of H. pylori in enrichment media.

* Corresponding author. Mailing address: Center for Food Safety and Quality Enhancement, University of Georgia, 1109 Experiment St., Griffin, GA 30223-1797. Phone: (770) 228-7284. Fax: (770) 229-3216. E-mail: MDOYLE{at}CFSQE.GRIFFIN.PEACHNET.EDU.

Journal of Clinical Microbiology, May 2000, p. 1984-1987, Vol. 38, No. 5
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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