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Journal of Clinical Microbiology, May 2000, p. 1992-1995, Vol. 38, No. 5
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Quantitation of Cell-Free and Cell-Associated Kaposi's Sarcoma-Associated Herpesvirus DNA by Real-Time PCR

Irene E. White and Thomas B. Campbell*

Division of Infectious Diseases, Department of Medicine, University of Colorado Health Sciences Center, Denver, Colorado

Received 13 October 1999/Returned for modification 14 January 2000/Accepted 21 February 2000

A real-time PCR assay for quantitation of Kaposi's sarcoma-associated herpes virus (KSHV or human herpesvirus 8) DNA was evaluated. The linear dynamic range was 10 to 105 copies of KSHV DNA (r2 > 0.99). The accuracy of DNA purification and quantitation was less than ±0.4 log10 copies for samples that contained from 10 to 105 copies of KSHV DNA. Cell-associated KSHV DNA was quantitated over a range of infected cell frequencies from 0.1 to 10-5, and cell-free KSHV DNA in plasma was quantitated over a range of 100 to 106 copies/ml. Real-time PCR provides a convenient method for quantitation of cell-free and cell-associated KSHV DNA in laboratory and clinical specimens.

* Corresponding author. Mailing address: Campus Box B-168, UCHSC, 4200 East Ninth Ave., Denver, CO 80262. Phone: (303) 315-8311. Fax: (303) 315-8681. E-mail: thomas.campbell{at}uchsc.edu.

Journal of Clinical Microbiology, May 2000, p. 1992-1995, Vol. 38, No. 5
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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