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Journal of Clinical Microbiology, June 2000, p. 2170-2173, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Evaluation of Three Rapid Methods for Detection of
Methicillin Resistance in Staphylococcus aureus
L.
Louie,*
S. O.
Matsumura,
E.
Choi,
M.
Louie, and
A. E.
Simor
Department of Microbiology, SD Laboratory
Services, Sunnybrook and Women's College Health Sciences Centre,
and the University of Toronto, Toronto, Ontario M4N 3M5, Canada
Received 24 November 1999/Returned for modification 10 February
2000/Accepted 6 March 2000
The probe-based Velogene Rapid MRSA Identification Assay (ID
Biomedical Corp., Vancouver, British Columbia, Canada) and the latex agglutination MRSA-Screen (Denka Seiken Co., Tokyo, Japan) were
evaluated for their ability to identify methicillin-resistant Staphylococcus aureus (MRSA) and to distinguish strains of
MRSA from borderline oxacillin-resistant S. aureus (BORSA;
mecA-negative, oxacillin MICs of 2 to 8 µg/ml). The
Velogene is a 90-min assay using a chimeric probe to detect the
mecA gene. MRSA-Screen is a 15-min latex agglutination test
with penicillin-binding protein 2a antibody-sensitized latex particles.
We compared these assays with the BBL Crystal MRSA ID System (Becton
Dickinson, Cockeysville, Md.) and with PCR for mecA gene
detection. A total of 397 clinical isolates of S. aureus
were tested, consisting of 164 methicillin-susceptible strains, 197 MRSA strains, and 37 BORSA strains. All assays performed well for the
identification of MRSA with sensitivities and specificities for
Velogene, MRSA-Screen, and BBL Crystal MRSA ID of 98.5 and 100%, 98.5 and 100%, and 98.5 and 98%, respectively. Three MRSA strains were not
correctly identified by each of the Velogene and MRSA-Screen assays,
but repeat testing with a larger inoculum resolved the discrepancies.
The BBL Crystal MRSA ID test misclassified four BORSA strains as MRSA.
Both the Velogene and the MRSA-Screen assays are easy to perform, can
accurately differentiate BORSA isolates from MRSA isolates, and
provide a rapid alternative for the detection of methicillin resistance
in S. aureus in clinical laboratories, especially when
mecA PCR gene detection is unavailable.
*
Corresponding author. Mailing address: Department
of Microbiology, Sunnybrook and Women's College Health Sciences
Centre, B121-2075 Bayview Ave., North York, Ontario, Canada M4N 3M5.
Phone: 416-480-4242. Fax: 416-480-6845. E-mail:
llouie{at}srcl.sunnybrook.utoronto.ca.
Journal of Clinical Microbiology, June 2000, p. 2170-2173, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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