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Journal of Clinical Microbiology, June 2000, p. 2186-2190, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Comparison of Pulsed-Field Gel Electrophoresis and Coagulase Gene Restriction Profile Analysis Techniques in the Molecular Typing of Staphylococcus aureus

Chien-Shun Chiou,* Hsiao-Lun Wei, and Li-Chu Yang

The Third Branch Office, Center for Disease Control, Department of Health, Taichung City, Taiwan

Received 4 October 1999/Returned for modification 10 November 1999/Accepted 22 March 2000

Pulsed-field gel electrophoresis (PFGE) and coagulase gene restriction profile (CRP) analysis techniques were used to analyze 71 Staphylococcus aureus isolates recovered from nine food-borne disease outbreaks. Twenty-two PFGE profiles and 11 CRPs were identified, with discrimination indices of 0.86 and 0.72, respectively. In addition, the variable regions of the coagulase genes of 39 isolates were sequenced and showed extensive identity, indicating that this is not an efficient alternative for the molecular typing of S. aureus.

* Corresponding author. Mailing address: 4F 103 Minchuan Road, Taichung City 403, Taiwan. Phone: 886-4-2225196. Fax: 886-4-2221917. E-mail address: nipmcsc{at}cdc.gov.tw.

Journal of Clinical Microbiology, June 2000, p. 2186-2190, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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