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Journal of Clinical Microbiology, June 2000, p. 2354-2361, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Mass Spectrometric Identification of Mtb81, a Novel Serological Marker for Tuberculosis

Ronald C. Hendrickson,1,* John F. Douglass,1 Lisa D. Reynolds,1 Patricia D. McNeill,1 Darrick Carter,1 Steven G. Reed,1,2,3 and Raymond L. Houghton1

Corixa Corporation1 and Infectious Disease Research Institute,2 Seattle Washington 98140, and Department of Pathobiology, University of Washington, Seattle, Washington 981953

Received 23 December 1999/Returned for modification 10 February 2000/Accepted 13 March 2000

We have used serological proteome analysis in conjunction with tandem mass spectrometry to identify and sequence a novel protein, Mtb81, which may be useful for the diagnosis of tuberculosis (TB), especially for patients coinfected with human immunodeficiency virus (HIV). Recombinant Mtb81 was tested by an enzyme-linked immunosorbent assay to detect antibodies in 25 of 27 TB patients (92%) seropositive for HIV as well as in 38 of 67 individuals (57%) who were TB positive alone. No reactivity was observed in 11 of 11 individuals (100%) who were HIV seropositive alone. In addition, neither sera from purified protein derivative (PPD)-negative (0 of 29) nor sera from healthy (0 of 45) blood donors tested positive with Mtb81. Only 2 of 57 of PPD-positive individuals tested positive with Mtb81. Sera from individuals with smear-positive TB and seropositive for HIV but who had tested negative for TB in the 38-kDa antigen immunodiagnostic assay were also tested for reactivity against Mtb81, as were sera from individuals with lung cancer and pneumonia. Mtb81 reacted with 26 of 37 HIV+ TB+ sera (70%) in this group, compared to 2 of 37 (5%) that reacted with the 38-kDa antigen. Together, these results demonstrate that Mtb81 may be a promising complementary antigen for the serodiagnosis of TB.

* Corresponding author. Present address: Protana A/S, Staermosegaardsvej 16, DK-5230 Odense M, Denmark. Phone: 45 63 15 20 30. E-mail: hendrickson{at}protana.com.

Journal of Clinical Microbiology, June 2000, p. 2354-2361, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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