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Journal of Clinical Microbiology, June 2000, p. 2378-2380, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

New Chromogenic Identification and Detection of Staphylococcus aureus and Methicillin-Resistant S. aureus

John Merlino,1,2,3,* Marcel Leroi,1 Ross Bradbury,1 Duncan Veal,2 and Colin Harbour3

Department of Microbiology and Infectious Diseases, Concord Repatriation General Hospital, Concord 2139,1 Department of Biological Sciences, Macquarie University, Concord 2109,2 and Department of Infectious Diseases, Faculty of Medicine, University of Sydney, 2006 Sydney,3 New South Wales, Australia

Received 13 September 1999/Returned for modification 13 November 1999/Accepted 15 February 2000

This paper describes a new chromogenic plate medium, CHROMagar Staph aureus (CHROMagar, Paris, France), for the identification of Staphylococcus aureus on the basis of colony pigmentation. The abilities of CHROMagar Staph aureus, thermostable nuclease (DNase), and mannitol salt agar (MSA) to identify S. aureus isolates (n = 114) and discriminate between S. aureus and coagulase-negative staphylococci (CoNS; n = 22) were compared. CHROMagar Staph aureus proved to be more sensitive and specific than DNase and MSA, allowing a reliable, simple, and rapid method for the identification of S. aureus isolates. All CoNS encountered in this study with the exception of S. chromogenes could be easily differentiated from S. aureus on this medium. The supplementation with 4 µg of oxacillin or methicillin per ml allowed simple identification of methicillin resistance in hospital-acquired S. aureus strains which show multiple-drug resistance profiles. Community-acquired methicillin-resistant S. aureus strains showing non-multi-drug resistance profiles require further evaluation on this new chromogenic medium. Methicillin or oxacillin resistance of all S. aureus isolates was confirmed by the detection of penicillin-binding protein 2a, encoded by the mecA gene, using the latex slide agglutination MRSA-Screen test (PBP 2' Test, DR900M; Oxoid).

* Corresponding author. Mailing address: Department of Microbiology and Infectious Diseases, Concord Repatriation General Hospital, Hospital Rd., Concord 2139, New South Wales, Australia. Phone: (612) 9767 6658. Fax: (612) 9767 7868. E-mail: john{at}micr.crg.cs.nsw.gov.au.

Journal of Clinical Microbiology, June 2000, p. 2378-2380, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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