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Journal of Clinical Microbiology, June 2000, p. 2392-2394, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Molecular Characterization of the vanD Gene Cluster and a Novel Insertion Element in a Vancomycin-Resistant Enterococcus Isolated in Canada

D. A. Boyd,1 J. Conly,2 H. Dedier,2 G. Peters,1 L. Robertson,1 E. Slater,1 and M. R. Mulvey1,*

Canadian Science Centre for Human and Animal Health, Bureau of Microbiology, Laboratory Centre for Disease Control, Health Canada, Winnipeg, Manitoba,1 and Department of Microbiology, The Toronto Hospital, University of Toronto, Toronto, Ontario,2 Canada

Received 16 August 1999/Returned for modification 24 January 2000/Accepted 15 March 2000

A single vanD-containing Enterococcus faecium strain (N97-330) was isolated in Canada. The vanD-containing region was cloned and sequenced. Although the proteins have more than 96% identity to a previously described vanD region in BM4339, the vanSD gene contains a frameshift mutation that leads to a predicted truncated protein. Furthermore, sequence analysis of the ddl gene revealed the presence of an IS982-like element (ISEfm1) which interrupted the D-Ala-D-Ala ligase. This suggested the constitutive expression of the vanD operon, which was confirmed. Pulsed-field gel electrophoresis fingerprinting demonstrated that BM4339 was not related to N97-330 (>15 band differences). Both strains contained multiple copies of the IS982-like element.

* Corresponding author. Mailing address: Canadian Science Centre for Human and Animal Health, Bureau of Microbiology, Laboratory Centre for Disease Control, Health Canada, 1015 Arlington St., Winnipeg, Manitoba, Canada R3E 3R2. Phone: (204) 789-2133. Fax: (204) 789-2018. E-mail: michael_mulvey{at}hc-sc.gc.ca.

Journal of Clinical Microbiology, June 2000, p. 2392-2394, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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