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Journal of Clinical Microbiology, June 2000, p. 2392-2394, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Molecular Characterization of the vanD
Gene Cluster and a Novel Insertion Element in a Vancomycin-Resistant
Enterococcus Isolated in Canada
D. A.
Boyd,1
J.
Conly,2
H.
Dedier,2
G.
Peters,1
L.
Robertson,1
E.
Slater,1 and
M.
R.
Mulvey1,*
Canadian Science Centre for Human and Animal
Health, Bureau of Microbiology, Laboratory Centre for Disease Control,
Health Canada, Winnipeg, Manitoba,1 and
Department of Microbiology, The Toronto Hospital,
University of Toronto, Toronto, Ontario,2 Canada
Received 16 August 1999/Returned for modification 24 January
2000/Accepted 15 March 2000
A single vanD-containing Enterococcus
faecium strain (N97-330) was isolated in Canada. The
vanD-containing region was cloned and sequenced.
Although the proteins have more than 96% identity to a previously
described vanD region in BM4339, the
vanSD gene contains a frameshift mutation that
leads to a predicted truncated protein. Furthermore, sequence analysis
of the ddl gene revealed the presence of an
IS982-like element (ISEfm1) which interrupted the D-Ala-D-Ala ligase. This suggested the
constitutive expression of the vanD operon, which was
confirmed. Pulsed-field gel electrophoresis fingerprinting demonstrated
that BM4339 was not related to N97-330 (>15 band differences).
Both strains contained multiple copies of the IS982-like element.
*
Corresponding author. Mailing address: Canadian
Science Centre for Human and Animal Health, Bureau of Microbiology,
Laboratory Centre for Disease Control, Health Canada, 1015 Arlington
St., Winnipeg, Manitoba, Canada R3E 3R2. Phone: (204) 789-2133. Fax: (204) 789-2018. E-mail: michael_mulvey{at}hc-sc.gc.ca.
Journal of Clinical Microbiology, June 2000, p. 2392-2394, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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