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Journal of Clinical Microbiology, June 2000, p. 2395-2397, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Comparison of Recoveries of Mycobacterium tuberculosis Using the Automated BACTEC MGIT 960 System, the BACTEC 460 TB System, and Löwenstein-Jensen Medium

Ákos Somoskövi,* Csaba Ködmön, Ákos Lantos, Zoltán Bártfai, Lilla Tamási, Judit Füzy, and Pál Magyar

Department of Respiratory Medicine, Semmelweis University, H-1536 Budapest 114, Hungary

Received 12 October 1999/Returned for modification 29 January 2000/Accepted 16 February 2000

Using two different liquid media and one conventional solid medium, a total of 57 mycobacterial isolates (Mycobacterium tuberculosis, n = 55; nontuberculous mycobacteria, n = 2) were recovered from 377 clinical specimens. The rates of recovery of M. tuberculosis were 96.4% with the BACTEC MGIT 960 liquid medium, 92.7% with BACTEC 12B liquid medium, and 81.8% with the Löwenstein-Jensen (LJ) medium. The mean time to detection of M. tuberculosis in smear-positive specimens was 12.6 days for BACTEC MGIT 960 medium, 13.8 days for BACTEC 12B medium, and 20.1 days for LJ medium, and in smear-negative specimens it was 15.8 days for BACTEC MGIT 960 medium, 17.7 days for BACTEC 12B medium, and 42.2 days for LJ medium. The rates of contamination were 3.7, 2.9, and 1.2% for the BACTEC MGIT 960, BACTEC 12B, and LJ media, respectively. In conclusion, the nonradiometric, fully automated 7-ml BACTEC MGIT 960 system can be considered a viable alternative to the semiautomated, radiometric BACTEC 460 TB system.

* Corresponding author. Mailing address: Department of Respiratory Medicine, Laboratory for Mycobacteria, Semmelweis Medical School, P.O. Box 250, H-1536 Budapest 114, Hungary. Phone: 36-1-355-8682. Fax: 36-1-214-2498. E-mail: akos{at}pulm.sote.hu.

Journal of Clinical Microbiology, June 2000, p. 2395-2397, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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