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Journal of Clinical Microbiology, June 2000, p. 2409-2411, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Limitations of Vitek GPS-418 Cards in Exact Detection of Vancomycin-Resistant Enterococci with the vanB Genotype

Tadashi Okabe,1 Kozue Oana,2 Yoshiyuki Kawakami,2,* Masaru Yamaguchi,1 Yuko Takahashi,1 Yukie Okimura,1 Takayuki Honda,3 and Tsutomu Katsuyama3

Central Clinical Laboratories, Shinshu University Hospital,1 Division of Clinical Microbiology, Department of Medical Technology, School of Allied Medical Sciences, Shinshu University,2 and Department of Laboratory Medicine, Shinshu University School of Medicine,3 Matsumoto, Nagano, 390-8621, Japan

Received 8 November 1999/Returned for modification 11 January 2000/Accepted 21 March 2000

The susceptibilities of 20 strains of vancomycin-resistant enterococci (VRE) with the vanB genotype obtained by using Vitek GPS-418 cards were compared with those obtained by the broth dilution method of the National Committee for Clinical Laboratory Standards (NCCLS) (approved standard M7-A4) and with those obtained by the agar screen method using bile esculin azide agar containing 6 µg of vancomycin per ml. Although both the broth dilution and agar screen methods disclosed no discordance, Vitek GPS-418 cards yielded a very major error compared with the results obtained by the reference broth dilution method of the NCCLS. Vitek GPS-418 cards were therefore found to have considerable room for improvement for the accurate detection of vanB VRE strains.

* Corresponding author. Mailing address: Division of Clinical Microbiology, Department of Medical Technology, School of Allied Medical Sciences, Shinshu University, Matsumoto 390-8621, Japan. Phone: 81-263-37-2381. Fax: 81-263-37-2370. E-mail: yk23724{at}gipac.shinshu-u.ac.jp.

Journal of Clinical Microbiology, June 2000, p. 2409-2411, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Abele-Horn, M., Hommers, L., Trabold, R., Frosch, M. (2006). Validation of VITEK 2 Version 4.01 Software for Detection, Identification, and Classification of Glycopeptide-Resistant Enterococci. J. Clin. Microbiol. 44: 71-76 [Abstract] [Full Text]  


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