JCM Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Okabe, T.
Right arrow Articles by Katsuyama, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Okabe, T.
Right arrow Articles by Katsuyama, T.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, June 2000, p. 2409-2411, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Limitations of Vitek GPS-418 Cards in Exact Detection of Vancomycin-Resistant Enterococci with the vanB Genotype

Tadashi Okabe,1 Kozue Oana,2 Yoshiyuki Kawakami,2,* Masaru Yamaguchi,1 Yuko Takahashi,1 Yukie Okimura,1 Takayuki Honda,3 and Tsutomu Katsuyama3

Central Clinical Laboratories, Shinshu University Hospital,1 Division of Clinical Microbiology, Department of Medical Technology, School of Allied Medical Sciences, Shinshu University,2 and Department of Laboratory Medicine, Shinshu University School of Medicine,3 Matsumoto, Nagano, 390-8621, Japan

Received 8 November 1999/Returned for modification 11 January 2000/Accepted 21 March 2000

The susceptibilities of 20 strains of vancomycin-resistant enterococci (VRE) with the vanB genotype obtained by using Vitek GPS-418 cards were compared with those obtained by the broth dilution method of the National Committee for Clinical Laboratory Standards (NCCLS) (approved standard M7-A4) and with those obtained by the agar screen method using bile esculin azide agar containing 6 µg of vancomycin per ml. Although both the broth dilution and agar screen methods disclosed no discordance, Vitek GPS-418 cards yielded a very major error compared with the results obtained by the reference broth dilution method of the NCCLS. Vitek GPS-418 cards were therefore found to have considerable room for improvement for the accurate detection of vanB VRE strains.

* Corresponding author. Mailing address: Division of Clinical Microbiology, Department of Medical Technology, School of Allied Medical Sciences, Shinshu University, Matsumoto 390-8621, Japan. Phone: 81-263-37-2381. Fax: 81-263-37-2370. E-mail: yk23724{at}gipac.shinshu-u.ac.jp.

Journal of Clinical Microbiology, June 2000, p. 2409-2411, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 2000 by the American Society for Microbiology. All rights reserved.