This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gronowski, A. M.
Right arrow Articles by Murray, P. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gronowski, A. M.
Right arrow Articles by Murray, P. R.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, June 2000, p. 2416-2418, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Reproducibility Problems with the Abbott Laboratories LCx Assay for Chlamydia trachomatis and Neisseria gonorrhoeae

Ann M. Gronowski,1,* Susan Copper,2 David Baorto,1,dagger and Patrick R. Murray1,Dagger

Department of Pathology, Division of Laboratory Medicine, Washington University School of Medicine,1 and Barnes-Jewish Hospital,2 St. Louis, Missouri 63110

Received 7 December 1999/Returned for modification 3 March 2000/Accepted 13 March 2000

This study demonstrates that significant reproducibility problems can occur during routine use of the Abbott Laboratories LCx assay for Chlamydia trachomatis and Neisseria gonorrhoeae. These problems can go undetected by the quality control procedures outlined in the manufacturer's package insert. We outline here procedures for detecting and preventing contamination and reproducibility problems.

* Corresponding author. Mailing address: Department of Pathology, Box 8118, Washington University School of Medicine, 660 South Euclid, St. Louis, MO 63110. Phone: (314) 362-0194. Fax: (314) 362-1461. E-mail: gronowski{at}pathology.wustl.edu.

dagger Present address: Department of Medical Informatics, Columbia University Medical Center, New York, N.Y.

Dagger Present address: Department of Pathology, University of Maryland, Baltimore, Md.

Journal of Clinical Microbiology, June 2000, p. 2416-2418, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Heystek, M, Ross, J D C, for the PID Study Group, (2009). A randomized double-blind comparison of moxifloxacin and doxycycline/metronidazole/ciprofloxacin in the treatment of acute, uncomplicated pelvic inflammatory disease. Int J STD AIDS 20: 690-695 [Abstract] [Full Text]  
  • Munson, E., Boyd, V., Czarnecka, J., Griep, J., Lund, B., Schaal, N., Hryciuk, J. E. (2007). Evaluation of Gen-Probe APTIMA-Based Neisseria gonorrhoeae and Chlamydia trachomatis Confirmatory Testing in a Metropolitan Setting of High Disease Prevalence. J. Clin. Microbiol. 45: 2793-2797 [Abstract] [Full Text]  
  • Schachter, J., Chow, J. M., Howard, H., Bolan, G., Moncada, J. (2006). Detection of Chlamydia trachomatis by Nucleic Acid Amplification Testing: Our Evaluation Suggests that CDC-Recommended Approaches for Confirmatory Testing Are Ill-Advised.. J. Clin. Microbiol. 44: 2512-2517 [Abstract] [Full Text]  
  • Chalker, V. J., Vaughan, H., Patel, P., Rossouw, A., Seyedzadeh, H., Gerrard, K., James, V. L. A. (2005). External Quality Assessment for Detection of Chlamydia trachomatis. J. Clin. Microbiol. 43: 1341-1347 [Abstract] [Full Text]  
  • Schachter, J., Hook, E. W., Martin, D. H., Willis, D., Fine, P., Fuller, D., Jordan, J., Janda, W. M., Chernesky, M. (2005). Confirming Positive Results of Nucleic Acid Amplification Tests (NAATs) for Chlamydia trachomatis: All NAATs Are Not Created Equal. J. Clin. Microbiol. 43: 1372-1373 [Abstract] [Full Text]  
  • Culler, E. E., Caliendo, A. M., Nolte, F. S. (2003). Reproducibility of Positive Test Results in the BDProbeTec ET System for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae. J. Clin. Microbiol. 41: 3911-3914 [Abstract] [Full Text]  
  • Castriciano, S., Luinstra, K., Jang, D., Patel, J., Mahony, J., Kapala, J., Chernesky, M. (2002). Accuracy of Results Obtained by Performing a Second Ligase Chain Reaction Assay and PCR Analysis on Urine Samples with Positive or Near-Cutoff Results in the LCx Test for Chlamydia trachomatis. J. Clin. Microbiol. 40: 2632-2634 [Abstract] [Full Text]  
  • Mallinson, H, Hopwood, J, Mutton, K (2002). Resolution of the recent performance problem of Abbott LCx Chlamydia trachomatis assay. Issues of repeat testing for confirmation of chlamydial infection. Sex. Transm. Infect. 78 : 225-226 [Full Text]  
  • Hammerschlag, M. R (2001). Use of nucleic acid amplification tests in investigating child sexual abuse. Sex. Transm. Infect. 77: 153-154 [Full Text]  
  • Van Dyck, E., Ieven, M., Pattyn, S., Van Damme, L., Laga, M. (2001). Detection of Chlamydia trachomatis and Neisseria gonorrhoeae by Enzyme Immunoassay, Culture, and Three Nucleic Acid Amplification Tests. J. Clin. Microbiol. 39: 1751-1756 [Abstract] [Full Text]  
  • Hanson, K. L., Cartwright, C. P. (2001). Evaluation of an Automated Liquid-Handling System (Tecan Genesis RSP 100) in the Abbott LCx Assay for Chlamydia trachomatis. J. Clin. Microbiol. 39: 1975-1977 [Abstract] [Full Text]  
  • Van Der Pol, B., Ferrero, D. V., Buck-Barrington, L., Hook, E. III, Lenderman, C., Quinn, T., Gaydos, C. A., Lovchik, J., Schachter, J., Moncada, J., Hall, G., Tuohy, M. J., Jones, R. B. (2001). Multicenter Evaluation of the BDProbeTec ET System for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae in Urine Specimens, Female Endocervical Swabs, and Male Urethral Swabs. J. Clin. Microbiol. 39: 1008-1016 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»