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Journal of Clinical Microbiology, June 2000, p. 2427-2428, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Limitation of the AccuProbe Coccidioides immitis Culture Identification Test: False-Negative Results with Formaldehyde-Killed Cultures

Sally G. Gromadzki1 and Vishnu Chaturvedi1,2,*

Mycology Laboratory, Wadsworth Center, New York State Department of Health, Albany, New York 12201-2002,1 and School of Public Health, State University of New York at Albany, Albany, New York 12201-05062

Received 28 December 1999/Returned for modification 31 January 2000/Accepted 9 March 2000

The AccuProbe Coccidioides immitis culture identification test (CI test) yielded false-negative results with formaldehyde-killed C. immitis submitted to a reference laboratory. Further evaluation with pure or mixed cultures or stored, heat-killed cultures revealed the CI test to be highly sensitive and specific for C. immitis except when the cultures were pretreated with formaldehyde.

* Corresponding author. Mailing address: Mycology Laboratory, Wadsworth Center, New York State Department of Health, 120 New Scotland Ave., Albany, NY 12201-2002. Phone: (518) 474-4177. Fax: (518) 486-7971. E-mail: vishnu{at}wadsworth.org.

Journal of Clinical Microbiology, June 2000, p. 2427-2428, Vol. 38, No. 6
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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