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Journal of Clinical Microbiology, July 2000, p. 2504-2511, Vol. 38, No. 7
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Genomic Variability of Haemophilus influenzae Isolated from Mexican Children Determined by Using Enterobacterial Repetitive Intergenic Consensus Sequences and PCR

Patricia Gomez-De-Leon,1 Jose I. Santos,2 Javier Caballero,3 Demostenes Gomez,4 Luz E. Espinosa,4 Isabel Moreno,5 Daniel Piñero,6 and Alejandro Cravioto1,*

Departamentos de Salud Publica1 y Medicina Experimental,2 Facultad de Medicina, Jardin Botanico, Instituto de Biologia,3 and Instituto de Ecologia,6 Universidad Nacional, Autonoma de Mexico, Hospital Infantil de Mexico "Federico Gomez,4 and Instituto Nacional de Diagnostico y Referencia Epidemiologicos,5 Mexico D.F., Mexico

Received 1 October 1999/Returned for modification 7 December 1999/Accepted 21 April 2000

Genomic fingerprints from 92 capsulated and noncapsulated strains of Haemophilus influenzae from Mexican children with different diseases and healthy carriers were generated by PCR using the enterobacterial repetitive intergenic consensus (ERIC) sequences. A cluster analysis by the unweighted pair-group method with arithmetic averages based on the overall similarity as estimated from the characteristics of the genomic fingerprints, was conducted to group the strains. A total of 69 fingerprint patterns were detected in the H. influenzae strains. Isolates from patients with different diseases were represented by a variety of patterns, which clustered into two major groups. Of the 37 strains isolated from cases of meningitis, 24 shared patterns and were clustered into five groups within a similarity level of 1.0. One fragment of 1.25 kb was common to all meningitis strains. H. influenzae strains from healthy carriers presented fingerprint patterns different from those found in strains from sick children. Isolates from healthy individuals were more variable and were distributed differently from those from patients. The results show that ERIC-PCR provides a powerful tool for the determination of the distinctive pathogenicity potentials of H. influenzae strains and encourage its use for molecular epidemiology investigations.

* Corresponding author. Mailing address: Apartado Postal 70-443, Mexico D.F. 04510, Mexico. Phone: (525)-6232401. Fax: (525)-6161616. E-mail: acq{at}servidor.unam.mx.

Journal of Clinical Microbiology, July 2000, p. 2504-2511, Vol. 38, No. 7
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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