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Journal of Clinical Microbiology, July 2000, p. 2546-2549, Vol. 38, No. 7
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Helicobacter canadensis sp. nov. Isolated from Humans with Diarrhea as an Example of an Emerging Pathogen

James G. Fox,1,* Chih Ching Chien,1 Floyd E. Dewhirst,2 Bruce J. Paster,2 Zeli Shen,1 Pasquale L. Melito,3 David L. Woodward,3 and Frank G. Rodgers3

Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge,1 and Forsyth Institute, Boston,2 Massachusetts, and National Laboratory for Enteric Pathogens, Winnipeg, Canada3

Received 18 February 2000/Returned for modification 28 March 2000/Accepted 11 April 2000

We recently analyzed 11 helicobacter isolates cultured from diarrhea patients in Canada. These isolates had been characterized biochemically by restriction fragment length polymorphism (RFLP; AluI, HhaI) analysis and by fatty-acid analysis as Helicobacter pullorum. However, four of the isolates differed biochemically from H. pullorum by their inability to hydrolyze indoxyl acetate and their resistance to nalidixic acid. Using complete 16S rRNA analysis, we determined that these four strains clustered near H. pullorum but had a sequence difference of 2% and therefore represent a novel helicobacter, Helicobacter canadensis. This novel helicobacter could also be distinguished from H. pullorum by RFLP analysis using ApaLI. The number of novel Helicobacter spp. associated with gastrointestinal disease in humans and animals is rapidly increasing. There are now six Helicobacter spp. isolated from diarrheic humans, the other five being H. pullorum, H. canis, "H. rappini," H. fennelliae, and H. cinaedi. This finding highlights the importance of careful molecular analysis in addition to standard biochemical tests in identifying the increasing number of Helicobacter spp. isolated from humans and animals.

* Corresponding author. Mailing address: Division of Comparative Medicine, Massachusetts Institute of Technology, 77 Massachusetts Ave., Bldg. 16, Rm. 825C, Cambridge, MA 02139. Phone: (617) 253-1757. Fax: (617) 258-5708. E-mail: jgfox{at}mit.edu.

Journal of Clinical Microbiology, July 2000, p. 2546-2549, Vol. 38, No. 7
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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