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Journal of Clinical Microbiology, July 2000, p. 2557-2562, Vol. 38, No. 7
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Differentiation of Borrelia burgdorferi
Sensu Lato on the Basis of RNA Polymerase Gene (rpoB)
Sequences
Seung-Hyun
Lee,1
Bum-Joon
Kim,2
Jong-Hyun
Kim,1
Kyung-Hee
Park,1
Seo-Jeong
Kim,3 and
Yoon-Hoh
Kook4,*
Department of Microbiology, College of
Medicine, Konkuk University, Chungju, Chungchongbuk-Do
380-701,1 Department of Microbiology,
Cheju National University College of Medicine, Cheju-Do
690-756,2 Department of Pediatrics,
Pundang CHA General Hospital, Pochun CHA University College of
Medicine, Sungnam, Kyonggi-Do 463-670,3 and
Department of Microbiology and Institute of Endemic
Diseases, Medical Research Center, Seoul National University
College of Medicine, and Clinical Research Institute, Seoul
National University Hospital, Seoul
110-799,4 Korea
Received 8 November 1999/Returned for modification 14 January
2000/Accepted 12 April 2000
We determined the nucleotide sequences (329 bp) of the
rpoB DNAs from 22 reference strains of
Borrelia. No insertions or deletions were observed. Deduced
amino acid sequences of amplified rpoB DNA comprised 109 amino acid residues (N450 to M558
[Escherichia coli numbering]). All amino acid sequences
were identical with the exception of those of Borrelia
lusitaniae PotiB2 (T461
A) and B. bissettii DN127 (I498
V). Each species of B. burgdorferi sensu lato was differentiated as a distinct
entity in the phylogenetic tree constructed by a
neighbor-joining method. B. burgdorferi sensu lato
could be distinguished from B. turicatae and B. hermsii, which are associated with relapsing fever. Seventeen
Korean isolates could be identified by PCR-linked direct sequencing and
restriction analysis of the rpoB DNA. These results suggest
that rpoB DNA is useful for identification and
characterization of Borrelia. In addition, we developed the
rapid species identification method using the species-specific
primer sets based on rpoB gene sequences.
*
Corresponding author. Mailing address: Department of
Microbiology, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-gu, Seoul 110-799, Korea. Phone: (82) 2-740-8313. Fax: (82) 2-743-0881. E-mail:
yhkook{at}plaza.snu.ac.kr.
Journal of Clinical Microbiology, July 2000, p. 2557-2562, Vol. 38, No. 7
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
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