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Journal of Clinical Microbiology, July 2000, p. 2557-2562, Vol. 38, No. 7
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Differentiation of Borrelia burgdorferi Sensu Lato on the Basis of RNA Polymerase Gene (rpoB) Sequences

Seung-Hyun Lee,1 Bum-Joon Kim,2 Jong-Hyun Kim,1 Kyung-Hee Park,1 Seo-Jeong Kim,3 and Yoon-Hoh Kook4,*

Department of Microbiology, College of Medicine, Konkuk University, Chungju, Chungchongbuk-Do 380-701,1 Department of Microbiology, Cheju National University College of Medicine, Cheju-Do 690-756,2 Department of Pediatrics, Pundang CHA General Hospital, Pochun CHA University College of Medicine, Sungnam, Kyonggi-Do 463-670,3 and Department of Microbiology and Institute of Endemic Diseases, Medical Research Center, Seoul National University College of Medicine, and Clinical Research Institute, Seoul National University Hospital, Seoul 110-799,4 Korea

Received 8 November 1999/Returned for modification 14 January 2000/Accepted 12 April 2000

We determined the nucleotide sequences (329 bp) of the rpoB DNAs from 22 reference strains of Borrelia. No insertions or deletions were observed. Deduced amino acid sequences of amplified rpoB DNA comprised 109 amino acid residues (N450 to M558 [Escherichia coli numbering]). All amino acid sequences were identical with the exception of those of Borrelia lusitaniae PotiB2 (T461right-arrowA) and B. bissettii DN127 (I498right-arrowV). Each species of B. burgdorferi sensu lato was differentiated as a distinct entity in the phylogenetic tree constructed by a neighbor-joining method. B. burgdorferi sensu lato could be distinguished from B. turicatae and B. hermsii, which are associated with relapsing fever. Seventeen Korean isolates could be identified by PCR-linked direct sequencing and restriction analysis of the rpoB DNA. These results suggest that rpoB DNA is useful for identification and characterization of Borrelia. In addition, we developed the rapid species identification method using the species-specific primer sets based on rpoB gene sequences.

* Corresponding author. Mailing address: Department of Microbiology, Seoul National University College of Medicine, 28 Yongon-dong, Chongno-gu, Seoul 110-799, Korea. Phone: (82) 2-740-8313. Fax: (82) 2-743-0881. E-mail: yhkook{at}plaza.snu.ac.kr.

Journal of Clinical Microbiology, July 2000, p. 2557-2562, Vol. 38, No. 7
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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