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Journal of Clinical Microbiology, July 2000, p. 2643-2648, Vol. 38, No. 7
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Virulent Rough Filaments of Listeria monocytogenes from Clinical and Food Samples Secreting Wild-Type Levels of Cell-Free p60 Protein

Neil J. Rowan,1,* Alan A. G. Candlish,1 Andreas Bubert,2 John G. Anderson,1 Karl Kramer,3 and Jim McLauchlin4

Department of Bioscience and Biotechnology, University of Strathclyde, Glasgow,1 and Food Safety Microbiology Laboratory, Public Health Laboratory Service, Colindale, London,4 United Kingdom, and Microbiological Analytics, Merck KGaA, 64271 Darmstadt,2 and Department of Botany, Technical University Munich-Weihenstephan, 85350 Freising,3 Germany

Received 21 December 1999/Returned for modification 21 March 2000/Accepted 17 April 2000

Atypical rough cell filaments of Listeria monocytogenes (designated FR variants), isolated from clinical and food samples, form long filaments up to 96 µm in length and demonstrated wild-type levels of adherence, invasion, and cytotoxicity to human epithelial HEp-2, Caco-2, and HeLa cells. Unlike previously described avirulent rough mutants of L. monocytogenes that secrete diminished levels of the major extracellular protein p60 and that form long chains that consist of multiple cells of similar size (designated MCR variants), FR variants secreted wild-type or greater levels of p60. This study shows that virulent filamentous forms of L. monocytogenes occur in clinical and food environments and have atypical morphological characteristics compared to those of the wild-type form.

* Corresponding author. Mailing address: Department of Bioscience and Biotechnology, University of Strathclyde, Royal College Bldg., 204 George St., Glasgow G1 1XW, United Kingdom. Phone: 44 (0) 141 548 2531. Fax: 44 (0) 141 553 4124. E-mail: n.j.rowan{at}strath.ac.uk.

Journal of Clinical Microbiology, July 2000, p. 2643-2648, Vol. 38, No. 7
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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