This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Roosendaal, R.
Right arrow Articles by Kusters, J. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Roosendaal, R.
Right arrow Articles by Kusters, J. G.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, July 2000, p. 2661-2664, Vol. 38, No. 7
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Slaughter Pigs Are Commonly Infected by Closely Related but Distinct Gastric Ulcerative Lesion-Inducing Gastrospirilla

Robert Roosendaal,1,* Jan H. Vos,2 Thijs Roumen,2 René van Vugt,1 Giovanni Cattoli,1 Aldert Bart,1,dagger Henricus L. B. M. Klaasen,3 Ernst J. Kuipers,4 Christina M. J. E. Vandenbroucke-Grauls,1 and Johannes G. Kusters1

Department of Medical Microbiology and Infection Control, Vrije Universiteit, 1081 BT Amsterdam,1 Department of Gastroenterology, Academic Hospital Vrije Universiteit, 1081 HV Amsterdam,4 Animal Health Service, 5282 SC Boxtel,2 and Bacteriological R&D, Intervet International BV, 5831 AN Boxmeer,3 The Netherlands

Received 29 October 1999/Returned for modification 26 March 2000/Accepted 30 April 2000

An association between (unculturable) gastrospirillum-like organisms (GLO) and ulcerative lesions in the pars oesophagea in stomachs of swine has been claimed. In dogs GLO detected by microscopy may represent several Helicobacter species or subspecies. Therefore we investigated which Helicobacter spp. are present in stomachs of swine and their possible association with ulcerative lesions of the pars oesophagea. The presence of Helicobacter spp. in the antrum and pars oesophagea in 122 stomachs of slaughter swine was determined by microscopy (n = 122), by culture on selective and nonselective media (n = 112), and by a genus-specific 16S ribosomal DNA (rDNA) PCR (n = 80). GLO could not be cultured. Phylogenetic analysis of 43 16S rDNA fragments (out of 54 PCR-positive biopsy specimens) revealed the presence of Helicobacter heilmannii type 1 in 42 of them. This correlated with the presence of bacteria with GLO morphology. Helicobacter bilis 16S rDNA was amplified directly from one sample harboring bacteria with H. bilis morphology. The association between Helicobacter spp. and gastric lesions was investigated with a second group of 41 pigs with (n = 21 cases) or without (n = 20 controls) gastric lesions. Fifteen of the 21 cases were positive by PCR or microscopy, compared to 7 of 20 of the controls (P = 0.03). 16S rDNA sequence analysis of 7 of 14 PCR-positive cases revealed the presence of H. heilmannii type 1. Microscopy showed bacteria with GLO morphology. One sample (cases) was culture negative but PCR positive for Helicobacter pullorum-related 16S rDNA. In conclusion, our findings indicate that H. heilmannii type 1 is the predominant Helicobacter spp. in the stomachs of pigs and that its presence is associated with ulcerative lesions in the pars oesophagea.

* Corresponding author. Mailing address: Academic Hospital Vrije Universteit, Department of Medical Microbiology and Infection Control, De Boelelaan 1117, 1081 HV Amsterdam, The Netherlands. Phone: 31 20 4440488. Fax: 31 20 4440743. E-mail: r.roosendaal{at}azvu.nl.

dagger Present address: Department of Medical Microbiology, Academic Medical Center, 1100 DD Amsterdam, The Netherlands.

Journal of Clinical Microbiology, July 2000, p. 2661-2664, Vol. 38, No. 7
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Haesebrouck, F., Pasmans, F., Flahou, B., Chiers, K., Baele, M., Meyns, T., Decostere, A., Ducatelle, R. (2009). Gastric Helicobacters in Domestic Animals and Nonhuman Primates and Their Significance for Human Health. Clin. Microbiol. Rev. 22: 202-223 [Abstract] [Full Text]  
  • Baele, M., Decostere, A., Vandamme, P., Ceelen, L., Hellemans, A., Mast, J., Chiers, K., Ducatelle, R., Haesebrouck, F. (2008). Isolation and characterization of Helicobacter suis sp. nov. from pig stomachs. Int. J. Syst. Evol. Microbiol. 58: 1350-1358 [Abstract] [Full Text]  
  • Hellemans, A., Chiers, K., Maes, D., De Bock, M., Decostere, A., Haesebrouck, F., Ducatelle, R. (2007). Prevalence of 'Candidatus Helicobacter suis' in pigs of different ages. Vet Rec. 161: 189-192 [Abstract] [Full Text]  
  • Krakowka, S., Ellis, J. (2006). Reproduction of Severe Gastroesophageal Ulcers (GEU) in Gnotobiotic Swine Infected with Porcine Helicobacter pylori-like Bacteria.. Vet Pathol 43: 956-962 [Abstract] [Full Text]  
  • O'Rourke, J. L., Solnick, J. V., Neilan, B. A., Seidel, K., Hayter, R., Hansen, L. M., Lee, A. (2004). Description of 'Candidatus Helicobacter heilmannii' based on DNA sequence analysis of 16S rRNA and urease genes. Int. J. Syst. Evol. Microbiol. 54: 2203-2211 [Abstract] [Full Text]  
  • Harper, C. G., Feng, Y., Xu, S., Taylor, N. S., Kinsel, M., Dewhirst, F. E., Paster, B. J., Greenwell, M., Levine, G., Rogers, A., Fox, J. G. (2002). Helicobacter cetorum sp. nov., a Urease-Positive Helicobacter Species Isolated from Dolphins and Whales. J. Clin. Microbiol. 40: 4536-4543 [Abstract] [Full Text]  
  • Harper, C. M. G., Xu, S., Feng, Y., Dunn, J. L., Taylor, N. S., Dewhirst, F. E., Fox, J. G. (2002). Identification of Novel Helicobacter spp. from a Beluga Whale. Appl. Environ. Microbiol. 68: 2040-2043 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»