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Journal of Clinical Microbiology, July 2000, p. 2734-2737, Vol. 38, No. 7
Klinik für Innere Medizin m.S.
Hämatologie und Onkologie, Charité-Campus Virchow
Klinikum, Humboldt Universität zu
Berlin,1 TIB
Molbiol,2 and Department of
Virology, Robert Koch-Institut,3 Berlin,
Germany
Received 4 October 1999/Returned for modification 18 January
2000/Accepted 17 April 2000
A real-time PCR assay was developed to quantify human
cytomegalovirus (CMV) DNA. This assay was used to demonstrate a higher CMV DNA load in plasma of bone marrow transplant patients than in that
of blood donors. The CMV load was higher in CMV antigen-positive patients than in antigen-negative patients.
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.
Detection of Human Cytomegalovirus DNA by Real-Time
Quantitative PCR
*
Corresponding author. Mailing address: Klinik
für Innere Medizin m.S. Hämatologie und Onkologie,
Charité-Campus Virchow Klinikum, Humboldt Universität zu
Berlin, Augustenburger Platz 1, 13353 Berlin, Germany. Phone:
49-30-45053673. Fax: 49-30-45053925. E-mail:
wolfgang.siegert{at}charite.de.
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