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Journal of Clinical Microbiology, July 2000, p. 2734-2737, Vol. 38, No. 7
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Detection of Human Cytomegalovirus DNA by Real-Time Quantitative PCR

Andreas Nitsche,1 Nina Steuer,1 Christian Andreas Schmidt,1 Olfert Landt,2 Heinz Ellerbrok,3 Georg Pauli,3 and Wolfgang Siegert1,*

Klinik für Innere Medizin m.S. Hämatologie und Onkologie, Charité-Campus Virchow Klinikum, Humboldt Universität zu Berlin,1 TIB Molbiol,2 and Department of Virology, Robert Koch-Institut,3 Berlin, Germany

Received 4 October 1999/Returned for modification 18 January 2000/Accepted 17 April 2000

A real-time PCR assay was developed to quantify human cytomegalovirus (CMV) DNA. This assay was used to demonstrate a higher CMV DNA load in plasma of bone marrow transplant patients than in that of blood donors. The CMV load was higher in CMV antigen-positive patients than in antigen-negative patients.

* Corresponding author. Mailing address: Klinik für Innere Medizin m.S. Hämatologie und Onkologie, Charité-Campus Virchow Klinikum, Humboldt Universität zu Berlin, Augustenburger Platz 1, 13353 Berlin, Germany. Phone: 49-30-45053673. Fax: 49-30-45053925. E-mail: wolfgang.siegert{at}charite.de.

Journal of Clinical Microbiology, July 2000, p. 2734-2737, Vol. 38, No. 7
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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