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Journal of Clinical Microbiology, August 2000, p. 2853-2857, Vol. 38, No. 8
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

vacA Genotypes in Helicobacter pylori Strains Isolated from Children with and without Duodenal Ulcer in Brazil

Valquiria Ribeiro De Gusmão,1 Edilberto Nogueira Mendes,1,* Dulciene Maria De Magalhães Queiroz,2 Gifone Aguiar Rocha,2 Andreia Maria Camargos Rocha,2 Abdussalam Ali Ramadan Ashour,1 and Anfrisina Sales Teles Carvalho3

Laboratory of Molecular Biology,1 Laboratory of Research in Bacteriology,2 and Department of Pediatrics,3 Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil, 30130-100

Received 30 December 1999/Returned for modification 23 February 2000/Accepted 15 May 2000

Data concerning the association between vacA genotypes and disease in children in both developed and developing countries are scarce, especially because of the small number of children with a duodenal ulcer studied. The vacA genotypes of Helicobacter pylori strains obtained from 65 children (24 with a duodenal ulcer and 41 without a duodenal ulcer; 33 girls; mean age, 10.2 years; age range, 1 to 17 years) were investigated as described by J. C. Atherton et al. (J. Clin. Microbiol. 37:2979-2982, 1999). Ten (15.4%) children were infected with more than one H. pylori strain. None of these patients were included in our analysis of the relationship between gastric disorders and specific vacA genotypes. The s1 allele was detected in all H. pylori strains isolated from patients with a duodenal ulcer and from 21 (58.3%) patients without a duodenal ulcer (P = 0.003). Strains with the s2 allele were found only in patients without ulcer (n = 15; 41.7%). Most s1 strains had the s1b allele (97.5%), a result similar to that reported for adults from the Iberian peninsula, which could reflect the Brazilian population origin. One untypeable s1 strain was isolated. The m1 allele was also more frequently found in strains obtained from duodenal ulcer patients (P = 0.028). The m2 allele was found in strains obtained from 20 (36.4%) children, 3 (15.8%) with an ulcer and 17 (47.2%) without an ulcer. Only one m hybrid strain (m1 and m2 hybrid) was detected. It was demonstrated for the first time that the frequencies of colonization with strains with the s1 allele (14.3% in children up to 8 years of age and 85.7% in older patients; P = 0.012) and of strains with the m1 allele (11.1% in patients up to the age 8 years and 88.9% in older children; P = 0.013) increase with age.

* Corresponding author. Mailing address: Faculdade de Medicina/UFMG, Av. Alfredo Balena, 190-sala 6018, 30130-100, Belo Horizonte, Brazil. Phone: (55 31) 248 9775. Fax: (55 31) 248 9782. E-mail: enmendes{at}medicina.ufmg.br.

Journal of Clinical Microbiology, August 2000, p. 2853-2857, Vol. 38, No. 8
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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