This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Beggs, M. L.
Right arrow Articles by Cave, M. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Beggs, M. L.
Right arrow Articles by Cave, M. D.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, August 2000, p. 2923-2928, Vol. 38, No. 8
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Mapping of IS6110 Insertion Sites in Two Epidemic Strains of Mycobacterium tuberculosis

Marjorie L. Beggs,1,* Kathleen D. Eisenach,1,2 and M. Donald Cave3

Departments of Pathology,1 Anatomy,3 and Microbiology and Immunology,2 University of Arkansas for Medical Sciences and J. L. McClellan Memorial Veterans Hospital, Little Rock, Arkansas

Received 3 February 2000/Returned for modification 22 March 2000/Accepted 26 May 2000

A widely distributed strain designated 210 was identified in a study of the diversity of Mycobacterium tuberculosis DNA fingerprints from three geographically separate states in the United States. This strain is characterized by a 21-band fingerprint pattern when probed with IS6110, and the pattern is similar to that displayed by strains designated W. Intracellular growth of strain 210 isolates in human macrophages is significantly faster than that of isolates from other clusters or nonclustered isolates. The purpose of this study was to identify the sites of IS6110 insertions in strain 210 and compare these to IS6110 insertion sites in strain W. Our hypothesis is that an IS6110 insertion site(s) could possibly be responsible for a strain's increased capacity for transmission and/or replication. In this report, the insertion sites in strains 210 and W are described and referenced to their location in the M. tuberculosis H37Rv genome sequence. The W and 210 strains have 17 identical sites of IS6110 insertion and additional sequence not found in H37Rv but present in other clinical isolates. The IS6110 insertion site in the 36-bp direct repeat (DR) region of strains 210 and W has 15 spacers in the left flanking region. The DR region on the right side of IS6110 has been deleted. Five sites of insertion in strain 210 not found in strain W are described, as well as two unique sites in strain W. One copy of IS6110 was found to reside 55 bp in the ctpD gene. This gene is expressed, indicating that IS6110 can provide a promoter sequence for the transcription of genes.

* Corresponding author. Mailing address: John L. McClellan Memorial Veterans Hospital, Medical Research Department, Slot 151, Room GB 126, 4300 W. 7th St., Little Rock, AR 72205. Phone: (501) 257-4826. Fax: (501) 664-6748. E-mail: BeggsMarjorieL{at}exchange.uams.edu.

Journal of Clinical Microbiology, August 2000, p. 2923-2928, Vol. 38, No. 8
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Lazzarini, L. C. O., Huard, R. C., Boechat, N. L., Gomes, H. M., Oelemann, M. C., Kurepina, N., Shashkina, E., Mello, F. C. Q., Gibson, A. L., Virginio, M. J., Marsico, A. G., Butler, W. R., Kreiswirth, B. N., Suffys, P. N., Lapa e Silva, J. R., Ho, J. L. (2007). Discovery of a Novel Mycobacterium tuberculosis Lineage That Is a Major Cause of Tuberculosis in Rio de Janeiro, Brazil. J. Clin. Microbiol. 45: 3891-3902 [Abstract] [Full Text]  
  • Hanekom, M., van der Spuy, G. D., Streicher, E., Ndabambi, S. L., McEvoy, C. R. E., Kidd, M., Beyers, N., Victor, T. C., van Helden, P. D., Warren, R. M. (2007). A Recently Evolved Sublineage of the Mycobacterium tuberculosis Beijing Strain Family Is Associated with an Increased Ability to Spread and Cause Disease. J. Clin. Microbiol. 45: 1483-1490 [Abstract] [Full Text]  
  • Andre, M., Ijaz, K., Tillinghast, J. D., Krebs, V. E., Diem, L. A., Metchock, B., Crisp, T., McElroy, P. D. (2007). Transmission Network Analysis to Complement Routine Tuberculosis Contact Investigations. Am. J. Public Health 97: 470-477 [Abstract] [Full Text]  
  • Mathema, B., Kurepina, N. E., Bifani, P. J., Kreiswirth, B. N. (2006). Molecular Epidemiology of Tuberculosis: Current Insights. Clin. Microbiol. Rev. 19: 658-685 [Abstract] [Full Text]  
  • Ma, X., Wang, H., Deng, Y., Liu, Z., Xu, Y., Pan, X., Musser, J. M., Graviss, E. A. (2006). rpoB Gene Mutations and Molecular Characterization of Rifampin-Resistant Mycobacterium tuberculosis Isolates from Shandong Province, China.. J. Clin. Microbiol. 44: 3409-3412 [Abstract] [Full Text]  
  • Yesilkaya, H., Dale, J. W., Strachan, N. J. C., Forbes, K. J. (2005). Natural Transposon Mutagenesis of Clinical Isolates of Mycobacterium tuberculosis: How Many Genes Does a Pathogen Need?. J. Bacteriol. 187: 6726-6732 [Abstract] [Full Text]  
  • Mokrousov, I., Ly, H. M., Otten, T., Lan, N. N., Vyshnevskyi, B., Hoffner, S., Narvskaya, O. (2005). Origin and primary dispersal of the Mycobacterium tuberculosis Beijing genotype: Clues from human phylogeography. Genome Res 15: 1357-1364 [Abstract] [Full Text]  
  • Kurepina, N., Likhoshvay, E., Shashkina, E., Mathema, B., Kremer, K., van Soolingen, D., Bifani, P., Kreiswirth, B. N. (2005). Targeted Hybridization of IS6110 Fingerprints Identifies the W-Beijing Mycobacterium tuberculosis Strains among Clinical Isolates. J. Clin. Microbiol. 43: 2148-2154 [Abstract] [Full Text]  
  • Mokrousov, I., Narvskaya, O., Limeschenko, E., Vyazovaya, A. (2005). Efficient Discrimination within a Corynebacterium diphtheriae Epidemic Clonal Group by a Novel Macroarray-Based Method. J. Clin. Microbiol. 43: 1662-1668 [Abstract] [Full Text]  
  • Niobe-Eyangoh, S. N., Kuaban, C., Sorlin, P., Thonnon, J., Vincent, V., Gutierrez, M. C. (2004). Molecular Characteristics of Strains of the Cameroon Family, the Major Group of Mycobacterium tuberculosis in a Country with a High Prevalence of Tuberculosis. J. Clin. Microbiol. 42: 5029-5035 [Abstract] [Full Text]  
  • Warren, R. M., Victor, T. C., Streicher, E. M., Richardson, M., Beyers, N., van Pittius, N. C. G., van Helden, P. D. (2004). Patients with Active Tuberculosis often Have Different Strains in the Same Sputum Specimen. Am. J. Respir. Crit. Care Med. 169: 610-614 [Abstract] [Full Text]  
  • Soto, C. Y., Menendez, M. C., Perez, E., Samper, S., Gomez, A. B., Garcia, M. J., Martin, C. (2004). IS6110 Mediates Increased Transcription of the phoP Virulence Gene in a Multidrug-Resistant Clinical Isolate Responsible for Tuberculosis Outbreaks. J. Clin. Microbiol. 42: 212-219 [Abstract] [Full Text]  
  • Barnes, P. F., Cave, M. D. (2003). Molecular Epidemiology of Tuberculosis. NEJM 349: 1149-1156 [Full Text]  
  • Sampson, S. L., Warren, R. M., Richardson, M., Victor, T. C., Jordaan, A. M., van der Spuy, G. D., van Helden, P. D. (2003). IS6110-Mediated Deletion Polymorphism in the Direct Repeat Region of Clinical Isolates of Mycobacterium tuberculosis. J. Bacteriol. 185: 2856-2866 [Abstract] [Full Text]  
  • Gutacker, M. M., Smoot, J. C., Migliaccio, C. A. L., Ricklefs, S. M., Hua, S., Cousins, D. V., Graviss, E. A., Shashkina, E., Kreiswirth, B. N., Musser, J. M. (2002). Genome-Wide Analysis of Synonymous Single Nucleotide Polymorphisms in Mycobacterium tuberculosis Complex Organisms: Resolution of Genetic Relationships Among Closely Related Microbial Strains. Genetics 162: 1533-1543 [Abstract] [Full Text]  
  • Li, Q., Whalen, C. C., Albert, J. M., Larkin, R., Zukowski, L., Cave, M. D., Silver, R. F. (2002). Differences in Rate and Variability of Intracellular Growth of a Panel of Mycobacterium tuberculosis Clinical Isolates within a Human Monocyte Model. Infect. Immun. 70: 6489-6493 [Abstract] [Full Text]  
  • Weis, S. E., Pogoda, J. M., Yang, Z., Cave, M. D., Wallace, C., Kelley, M., Barnes, P. F. (2002). Transmission Dynamics of Tuberculosis in Tarrant County, Texas. Am. J. Respir. Crit. Care Med. 166: 36-42 [Abstract] [Full Text]  
  • Kivi, M., Liu, X., Raychaudhuri, S., Altman, R. B., Small, P. M. (2002). Determining the Genomic Locations of Repetitive DNA Sequences with a Whole-Genome Microarray: IS6110 in Mycobacterium tuberculosis. J. Clin. Microbiol. 40: 2192-2198 [Abstract] [Full Text]  
  • Mokrousov, I., Narvskaya, O., Otten, T., Limeschenko, E., Steklova, L., Vyshnevskiy, B. (2002). High Prevalence of KatG Ser315Thr Substitution among Isoniazid-Resistant Mycobacterium tuberculosis Clinical Isolates from Northwestern Russia, 1996 to 2001. Antimicrob. Agents Chemother. 46: 1417-1424 [Abstract] [Full Text]  
  • Mokrousov, I., Narvskaya, O., Limeschenko, E., Otten, T., Vyshnevskiy, B. (2002). Novel IS6110 Insertion Sites in the Direct Repeat Locus of Mycobacterium tuberculosis Clinical Strains from the St. Petersburg Area of Russia and Evolutionary and Epidemiological Considerations. J. Clin. Microbiol. 40: 1504-1507 [Abstract] [Full Text]  
  • Sampson, S., Warren, R., Richardson, M., van der Spuy, G., van Helden, P., Dunlap, N., Benjamin, W. H. Jr. (2001). IS6110 Insertions in Mycobacterium tuberculosis: Predominantly into Coding Regions. J. Clin. Microbiol. 39: 3423-3424 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»