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Journal of Clinical Microbiology, August 2000, p. 2989-2993, Vol. 38, No. 8
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Molecular Analysis of H Antigens Reveals that Human Diarrheagenic Escherichia coli O26 Strains That Carry the eae Gene Belong to the H11 Clonal Complex

Wen-Lan Zhang,1 Martina Bielaszewska,1,2 Jochen Bockemühl,3 Herbert Schmidt,1 Flemming Scheutz,4 and Helge Karch1,*

Institut für Hygiene und Mikrobiologie der Universität Würzburg, 97080 Würzburg,1 and Nationales Referenzzentrum für Salmonellen und andere bakterielle Enteritiserreger, Abteilung Bakteriologie, Hygiene Institut, 20539 Hamburg,3 Germany; Institute for Medical Microbiology, The 2nd Medical Faculty, Charles University, 150 06 Prague, Czech Republic2; and The International Escherichia and Klebsiella Centre (World Health Organization), Statens Serum Institut, 2300 Copenhagen, Denmark4

Received 23 February 2000/Returned for modification 22 April 2000/Accepted 29 April 2000

Fifty-seven Escherichia coli O26 strains isolated from patients in six countries were investigated by PCR restriction fragment length polymorphism (RFLP) analysis of the flagellin-encoding (fliC) gene (fliC RFLP analysis). The strains were determined by serotyping to belong to five different H types or were nonmotile. The fliC RFLP analysis revealed only two different patterns among the 57 strains. One fliC RFLP pattern was displayed by 54 strains and was identical to that of E. coli H11 reference strain Su4321-41. The other fliC RFLP pattern was observed for three strains and was identical to that for E. coli H32 reference strain K10. The 54 strains with the H11 fliC RFLP pattern included 22 strains of serotype O26:H11, 23 nonmotile strains, and 9 strains that were initially serotyped as H2, H8, H21, and H32 but that were confirmed to express H11 by repeat serotyping. All 54 strains with the H11 fliC RFLP pattern contained the attaching-and-effacing (eae) gene. The three strains with the H32 fliC RFLP pattern belonged to serotype O26:H32, and all were eae negative. The fliC genes of 14 selected E. coli O26:H11 strains isolated between 1964 and 1999 had identical nucleotide sequences. Our results demonstrate that E. coli O26 strains that carry the eae gene belong exclusively to the H11 clonal complex. Since there were no H11 fliC allelic variations among the O26 strains tested, E. coli O26:H11 may have emerged recently. The fliC PCR-RFLP test is a reliable, easy-to-perform, and rapid method for determination of the H types of E. coli O26 isolates.

* Corresponding author. Mailing address: Institut für Hygiene und Mikrobiologie der Universität Würzburg, Josef-Schneider-Str. 2, 97080 Würzburg, Germany. Phone: 49-931-201 5162. Fax: 49-931-201 5166. E-mail: hkarch{at}hygiene.uni-wuerzburg.de.

Journal of Clinical Microbiology, August 2000, p. 2989-2993, Vol. 38, No. 8
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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