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Journal of Clinical Microbiology, August 2000, p. 3068-3071, Vol. 38, No. 8
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

High-Resolution Genotyping of Chlamydia trachomatis from Recurrent Urogenital Infections

Lisbeth Nørum Pedersen,1,* Holger Olaf Kjær,2 Jens Kjølseth Møller,1 Torben Falck Ørntoft,3 and Lars Ostergaard4

Department of Clinical Microbiology, Aarhus Municipal Hospital,1 and Department of Infectious Diseases, Marselisborg Hospital,4 DK-8000 Aarhus, Department of Internal Medicine, Horsens County Hospital, DK-8700 Horsens,2 and Department of Clinical Biochemistry, Skejby Hospital, DK-8200 Aarhus N,3 Denmark

Received 13 January 2000/Returned for modification 14 March 2000/Accepted 18 May 2000

A method for Chlamydia trachomatis restriction fragment length polymorphism (RFLP) analysis and complete sequencing of omp1 was developed for use on samples collected at home, and results were compared. Genotyping by sequencing was superior to RFLP analysis. The omp1 gene in 31 clinical strains harbored few mutations compared to the same gene in ATCC reference strains. Follow-up samples obtained during a 24-week period from 31 patients showed recurrence with the same genotype in five cases and a new genotype in one case.

* Corresponding author. Mailing address: Department of Clinical Microbiology, Nørrebrogade 44, Århus Kommunehospital, DK-8000 Århus, Denmark. Phone: 45 89 49 35 26. Fax: 45 89 49 35 50. E-mail: Akh.grp02s.lhp{at}aaa.dk.

Journal of Clinical Microbiology, August 2000, p. 3068-3071, Vol. 38, No. 8
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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