This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Legrand, E.
Right arrow Articles by Rastogi, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Legrand, E.
Right arrow Articles by Rastogi, N.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, August 2000, p. 3080-3084, Vol. 38, No. 8
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

A Molecular Epidemiological Study of Mycobacterium simiae Isolated from AIDS Patients in Guadeloupe

Eric Legrand, Anne Devallois, Lionel Horgen, and Nalin Rastogi*

Unité de la Tuberculose et des Mycobactéries, Institut Pasteur, F-97165 Pointe à Pitre-Cedex, Guadeloupe

Received 28 April 2000/Accepted 18 May 2000

A molecular epidemiological study of Mycobacterium simiae strains isolated from AIDS patients in Guadeloupe was performed by the random amplification of polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) of DraI- or XbaI-digested bacterial DNAs. A comparison of RAPD profiles suggested a similarity of banding patterns within a group of patients (two clusters of two and three patients), but the available epidemiological and clinical information did not support this finding. PFGE, on the other hand, showed that all the patients were contaminated with individual isolates. Combined numerical analysis performed by compiling the PFGE patterns obtained after XbaI and DraI digestions of bacterial DNAs suggested the occurrence of polyclonal infection in three of nine patients. Our results do not support a common source of M. simiae infection in Guadeloupe.

* Corresponding author. Mailing address: Unité Tuberculose et Mycobactéries, Institut Pasteur, Morne Jolivière, BP 484, F-97165 Pointe-à-Pitre Cedex, Guadeloupe. Phone: 590-893-881. Fax: 590-893-880. E-mail: rastogi{at}ipagua.gp.

Journal of Clinical Microbiology, August 2000, p. 3080-3084, Vol. 38, No. 8
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Phillips, D R, Krishnan, H, Watson, J (2008). First UK report of successful treatment of Mycobacterium simiae and immune reconstitution inflammatory syndrome in an HIV-seropositive patient. Sex. Transm. Infect. 84: 271-272 [Abstract] [Full Text]  
  • van Ingen, J., Boeree, M. J., Dekhuijzen, P. N. R., van Soolingen, D. (2008). Clinical relevance of Mycobacterium simiae in pulmonary samples. Eur Respir J 31: 106-109 [Abstract] [Full Text]  
  • Vanitha, J. D., Venkatasubramani, R., Dharmalingam, K., Paramasivan, C. N. (2003). Large-Restriction-Fragment Polymorphism Analysis of Mycobacterium chelonae and Mycobacterium terrae Isolates. Appl. Environ. Microbiol. 69: 4337-4341 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»