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Journal of Clinical Microbiology, August 2000, p. 3092-3095, Vol. 38, No. 8
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.

Simple and Reliable Multiplex PCR Assay for Surveillance Isolates of Vancomycin-Resistant Enterococci

Reiko Kariyama,1,* Ritsuko Mitsuhata,1 Joseph W. Chow,2 Don B. Clewell,3 and Hiromi Kumon1

Section of Infection Control, Okayama University Hospital, and Department of Urology, Okayama University Medical School, Okayama 700-8558, Japan1; Research and Medical Service, John D. Dingell VA Medical Center, and Division of Infectious Diseases, Wayne State University School of Medicine, Detroit, Michigan 482012; and Department of Biologic and Materials Sciences, School of Dentistry, and Department of Microbiology/Immunology, School of Medicine, The University of Michigan, Ann Arbor, Michigan 481093

Received 9 March 2000/Returned for modification 11 May 2000/Accepted 20 May 2000

Conditions have been optimized for the use of a multiplex PCR for the detection of vancomycin-resistant enterococci in nosocomial surveillance specimens. Seven primer sets targeting the genes vanA, vanB, vanC1, vanC2/C3 Enterococcus faecalis-specific, Enterococcus faecium-specific, and rrs (16S rRNA) were used in one reaction tube. The PCR method developed in the present study is simple and reliable for the rapid characterization of vancomycin-resistant enterococci.

* Corresponding author. Mailing address: Department of Urology, Okayama University Medical School, 2-5-1 Shikata, Okayama 700-8558, Japan. Phone: +81-86-223-7151, ext. 7288. Fax: +81-86-231-3986. E-mail: kariyama{at}med.okayama-u.ac.jp.

Journal of Clinical Microbiology, August 2000, p. 3092-3095, Vol. 38, No. 8
0095-1137/00/$04.00+0
Copyright © 2000, American Society for Microbiology. All rights reserved.


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